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《Journal of Jimei University(Natural Science)》 2008-03
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Sequence Analysis of the Structural Protein Genes of a Porcine Reproductive and Respiratory Syndrome Virus Collected from Fujian Province Swine Herd

WU Guo-ping1,3,SU Wen-jin2,3,CAO Min-jie2,3(1.Fisheries College,Jimei University,Xiamen 361021,China;2.School of Biotechnology,Jimei University,Xiamen 361021,China;3.Key Laboratory of Science and Technology for Aquaculture andFood Safety(Jimei University),Fujian Province,Xiamen 361021,China)  
Porcine Peproductive and Respiratory Syndrome Virus(PRRSV) FJ-1 was isolated from a pig farm in Fujian Province of China during an outbreak of PRRS.We determined a 3188-nucleotide and predicted amino acid sequence of ORFs 2～7 gene encoding the structual proteins of FJ-1,and compared of the structural protein genes of FJ-1 with the PRRSV strains of 18 fully structural protein genes.It displayed 89.7 %～92.4 % and 54.9 % nucleotide identities to 17 North American genotype strains and European genotype Lelystad,respectively,while the deduced amino acids of ORFs 2～7 of FJ-1 showed 85.6 %～98.6 % and 53.2 %～78.2 % identities to them,respectively.The phylogenetic tree analysis showed that FJ-1 was most closely related to the North American genotype strains,but distantly related to the European genotype Lelystad.This study suggests that FJ-1 belongs to North American genotype.
【Fund】： 福建省科技攻关重点项目(2003N083);; 福建省自然科学基金项目(X0650078)
【CateGory Index】： S852.65
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