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《Journal of Jimei University(Natural Science)》 2010-04
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Cloning,Recombinant Expression and GTP-binding Activity Analysis of Shrimp Ran Gene

HAN Fang1,2,WANG Zhi-yong1,2 (1. Fisheries College,Jimei University,Xiamen 361021,China; 2. Key Laboratory of Science and Technology for Aquaculture and Food Safety (Jimei University),Fujian Province,Xiamen 361021,China)  
In previous studies,a cDNA fragment was found to be highly homologous with ras-related nuclear proteins (Ran proteins) by suppression subtractive hybridization (SSH) using WSSV-resistant shrimp. In this investigation,Ran gene was cloned from Marsupenaeus japonicus shrimp by RACE-PCR,the total cDNA was 1 441 base pair and the ORF of shrimp Ran gene contained 645 bp,which encoded 215 amino acids. This was the first Ran gene obtained from marine invertebrates. Then the gene was cloned in pGEX4T-2 and expressed in Escherichia coli BL21. After induction with IPTG at 37 ℃ for 6 h,the GST-Ran fusion protein (about 50 ku) was expressed and purified by glutathione-agarose beads. Based on protein domain analysis,the Ran protein was predicted to have a GTP/ATP binding motif. To test whether the Ran protein was endowed with GTPase activity,the GTP-binding assay was performed. The result revealed that the Ran protein had the GTP-binding activity.
【Fund】: 福建省农业科技重大项目(2008N2004)
【CateGory Index】: Q78
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 LI Chang-jian,LIU Jun,SHI Yao-hua,YANG Shu-ting,GUI Jian-fang 1 (State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology, the Chinese Academy of Sciences,Wuhan 430072,China);Full Length cDNA Cloning and Tissue Expression Specificity of Ran Gene in Color Crucian Carp[J];Zoological Research;2003-03
2 WU Wen-lin,DAI Cong-jie,YUAN Jian-jun(School of Chem.& Bioscience,Quanzhou Normal University,Fujian 362000,China);Cloning,Structure Analysis and Recombinant Expression of Shrimp Rab Gene[J];Journal of Quanzhou Normal University;2007-02
3 LI Chang-jian~(1,2),LIU Jun~1,GUI Jian-fang~1(1.Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;2.Department of Life Sciences and Chemical Engineering,Hunan University of Science and Engineering,Yongzhou 425006,China);cDNA cloning of Ran gene and analyses on its expression characterization and role in sperm nuclei decondensation in vitro in gibel carp,Carassius auratus gibelio[J];Journal of Fisheries of China;2006-03
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 SHI Yao-hua WANG Yan GU Zhi-feng WANG Ai-min SU Shu-ye (Ocean College,Hainan University,Key Laboratory of Tropic Biological Resources,Ministry of Education,Hainan Key Laboratory of Tropical Hydrobiology Technology,Experimental Teaching Center of Marine Biology,Haikou 570228);Cloning and expression character analysis of Rab7 in Dunker Pinctada martensii[J];Progress in Fishery Sciences;2010-02
2 LI Chang-jian~(1,2),LIU Jun~1,GUI Jian-fang~1(1.Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;2.Department of Life Sciences and Chemical Engineering,Hunan University of Science and Engineering,Yongzhou 425006,China);cDNA cloning of Ran gene and analyses on its expression characterization and role in sperm nuclei decondensation in vitro in gibel carp,Carassius auratus gibelio[J];Journal of Fisheries of China;2006-03
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
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