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《Journal of Jimei University(Natural Science)》 2010-04
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The Vitro Simulated Digestion of Carp Allergens Collagen

LIU Guang-ming1,2,ZHONG Yong-jia1,2,CAO Min-jie1,2, CAI Qiu-feng1,2,ZHANG Ling-jing1,2,SU Wen-jin1,2 (1. School of Biotechnology Engineering,Jimei University,Xiamen 361021,China; 2. Key Laboratory of Science and Technology for Aquaculture and Food Safety (Jimei University),Fujian Province,Xiamen 361021,China)  
Firstly,acid method was used to extract the collagen from the skin and muscle of carp. Then pepsin,trypsin and chymotrypsin were used to digest the extracted collagen at different conditions based on the simulated gastric fluid's and intestinal fluid's formula provided by US pharmacopoeia. And the digested products were detected the digestion stability and immunogenicity in the simulated gastric fluid and intestinal fluid by SDS-PAGE and Western blot. It was found that the collagen could be digested by pepsin at 15 min, and it still had a certain degree of immunogenicity after 1 h. The collagen could be digested by trypsin at 30 min,and it still had a certain degree of immunogenicity after 3 h. The collagen wasn't almost digested by chymotrypsin at 1 ~ 4 h,and it still had the immunogenicity after 4 h. These results suggested that the digestion stability and immunogenicity of carp collagen were the major cause to induce the food allergy.
【Fund】: 国家自然科学基金资助项目(30871947);; 福建省自然科学基金资助项目(2008J0067)
【CateGory Index】: S917.4
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Chinese Journal Full-text Database 2 Hits
1 CAI Qiu-feng1,DU Xue-li1,2,ZHANG Ling-jing1,SUN Le-chang1,SU Wen-jin1,CAO Min-jie1(1.College of Biotechnology,Jimei University,Xiamen 361021,China;2.College of Food Science,Shanghai Fisheries University,Shanghai 200090,China);Purification and study on the collagen from Sipunculidae[J];Journal of Oceanography in Taiwan Strait;2007-04
2 Chen Shenru Cai Yangpeng Zhou Qiong Zhang Qibiao Cao Minjie (School of Biotechnolog, Jimei University, Xiamen 361021);Study on Collagen from Shark Skin and Bone[J];Journal of Chinese Institute of Food Science and Technology;2006-01
Chinese Journal Full-text Database 10 Hits
1 ZHANG Qing-ning1,HU Ming2,ZHU Rong-sheng2,REN Xiang-quan3,WU Ying2,WANG Huai-zhong2,LIU Yu-qing2,WANG Shu-bai1(1.Qingdao Agricultural University,Qingdao 266109,China;2.Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Livestock Technology Company of Dilisi Group,Zhucheng 262216,China);Microbial Properties and Application of Superior Bacteria in Deep-litter Ecosystem for Piggery[J];Shandong Agricultural Sciences;2009-04
2 LIU Su-xia et al(Henan Normal University,Xinxiang,Henan 453007);Study on Optimization for RAPD Reaction System and Genetic Relationship in Punica granatum[J];Journal of Anhui Agricultural Sciences;2007-36
3 ZHANG Jin-zhou et al(Department of Animal Science,Henan Institute of Science and Technique,Xinxiang,Henan 453003);Analysis of Population Genetic Distance of Three Carps with RAPD Markers[J];Journal of Anhui Agricultural Sciences;2008-23
4 ZHANG Jin-zhou et al(Department of Animal Science,Henan Institute of Science and Technique,Xinxiang,Henan 453003);Analysis of Population Genetic Distance for Three Carps with RAPD Markers[J];Journal of Anhui Agricultural Sciences;2008-24
5 ZHANG Xiao-hua et al(Jiangsu Vocational College of Agriculture and Forestry,Jurong,Jiangsu 212400);Study on Polymorphism of Isolated Strain Producing α-amylase from Red Soil in Jiangxi Province[J];Journal of Anhui Agricultural Sciences;2008-35
6 TIAN Yu lou1,2, LIU Fu rong1, LIU Jie1,3, JIANG li1, LUO Yang1, ZHANG Xue1,*1.Department of Cell Biology,China Medical University,Shenyang 110001,P.R.China2.Stomatolagical Hospital,China Medical University,Shenyang 110001,P.R.China3.Experiment and Techn;Ectopic Expression of Cyclin G2 Inhibits Cell Proliferation in HeLa Cancer Cell Line[J];Chinese Journal of Cancer;2002-06
7 Chen Yu-Bao, Hou Jian, Fu Wei-Jun, Ding Si-Qi, Wang Dong-Xing, Yuan Zhen-Gang, Kong Xian-Tao Department of Hematology, Changzheng Hospital,The Second Military Medical University, Shanghai,200003, P.R.China;Mechanism of Arsenic Trioxide-Induced Cytotoxicity on Multiple Myeloma Cells[J];Chinese Journal of Cancer;2003-12
8 DENG Yan-Chun, WANG Ji-Cun, LIU Xin-Ping, JI Shao-Ping, YAO Li-Bo Department of Neurology, Xijing Hospital, The Fourth Military Medical University, Xi?蒺an, Shaanxi, 710032, P. R. China;Cloning Tumor-related Genes and Tumor Suppressor Genes in Glioma with Polymerase Chain Reaction-based Subtractive Hybridization[J];Chinese Journal of Cancer;2005-06
9 WANG Lin,LIU Yu,WEI Hong (Laboratory Animal Center, Third Military Medical University, Chongqing 400038 , China; Department of Life sciences, Southwest Normal University, Chongqing 400715, China);Study on Preparation of Type I Collagen with HPLC and Its Characterization[J];Amino Acids & Biotic Resources;2004-02
10 LI Shi-kun TAN Tian-wei(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China);Extraction of RNA from waste Penicillium biomass[J];Journal of Beijing University of Chemical Technology;2005-06
China Proceedings of conference Full-text Database 3 Hits
1 YE Yi-chun~(1,2) LIN Hai~(2,4) ZENG Rui~(3,4) DAN Nian-hua~(1,2) MI Zhen-jian~2 WANG Kang-jian~2 DAN Wei-hua~(1,4) (1.Key Laboratory of Leather Chemistry and Engineering,Ministry of Education,Sichuan University,Chengdu 610065,China; 2.Chengdu Bailejin Biological Technology Ltd.Co.,Chengdu 610041,China; 3.Light Industry and Textile and Food Engineering Institute,Sichuan University,Chengdu 610065,China; 4.The Research Center of Biomedicine Engineering of Sichuan University,Chengdu 610065,China);The preparation and characterization of collagen-chitosan composite membranes[A];[C];2007
2 ;低温炮制工艺对紫河车水溶性蛋白组成及其纤溶活性的影响[A];[C];2008
3 Xia Mengyun Yang FaLong Zhang Huanrong Yu Hua(College of Life Science and Technology,Southwest University for Nationalities,610041);Advances in Methods for Rapid Detection of Salmonella[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 HUANG Xiao-Chun, SU Xiu-rong, SU Yue-ping(Faculty of Life Science and Technology, Ningbo University, Ningbo 315211, China);Approximate Compositions of Perinereis aibuhitensis and Phasolosma esculenta[J];Fisheries Science;2005-06
2 HU Xiao-cong(College of Fisheries, Jimei University, Xiamen 361021, China);Some Trace Elements in Sipunculids[J];Fisheries Science;2005-06
3 GUO Yao,ZENG Ming-yong,CUI Wen-xuan(Food Engineering Department,Fishery College,Ocean University of China,Qingdao 266003,China);Aquatic Collagen and Active polyeptide[J];Fisheries Science;2006-02
4 Liu Qinghui, Wang Cali and Liu Congli (Yellow Sea Fisheries Research institute, Qingdao 266071);STUDIES ON PROPERITIES OF COLLAGEN FROM FISH SCALE[J];MARINE FISHERRIES RESEACH;2000-03
5 SHEN Xian rong, JIANG Ding wen, JIA Fu xing, et al. ( Marine Biology Research Center, Naval Medical Research Institute, Shanghai 200433, China);Study on anti-senescence effect of Sipunculus nudus preparation[J];Chinese Journal of Marine Drugs;2004-01
6 JIANG Ding-wen, SHENG Xian-rong, JIA Fu-xing, CHU Zhi-yong, LI Shu-lin(Marine Biology Research Center, Naval Medical Research Institute, Shanghai 200433, China);Nutrient analysis and immune regulation study on extract of Sipunculidae[J];Chinese Journal of Biochemical Pharmaceutics;2004-02
7 QIN Yu qing, LIU Cheng chu,WANG Zao (College of Food Science , Shanghai Fisheries University, Shanghai 200090, China);Recovery of the collagen from squid skin[J];Journal of Shanghai Fisheries University;2002-02
8 Jia Shirong (Biotechnology Research Center,Chinese Academy of Agricultural Sciences,Beijing 100081);Environment and Food Biosafety Assessment of Transgenic Plants[J];PROGRESS IN BIOTECHNOLOGY;1997-06
9 YU Guan-xin,LI Jun,MAO Shu-he;Examination of Common Inhaling Allergens in 444 Patients with Chronic Urticaria Followed by Desensitization Treatment[J];The Chinese Journal of Dermatovenereology;2004-02
10 LI Ben-li,CHEN Shu-hua,SONG Yan;Observing Analysis of 450 Cases of Chronic Urticaria on Mensurating of Allergic Reaction and on Treating of Desensitization[J];The Chinese Journal of Dermatovenereology;2004-04
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