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《Journal of Jinan University(Natural Science & Medicine Edition)》 2009-01
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Culture of insect cell Sf9 in four different kinds of bioreactor

XIE Qiu-ling1,ZHENG Yun-cheng1,LIAO Mei-de2(1.Institute of Bioengineering,Jinan University,Guangzhou 510632,China;2.Key Laboratory of Pesticide & Chemical Biology Ministry of Education South China Agricultural University,Guangzhou 510642,China)  
The insect cell baculovirus system has been increasingly used for the expression of a wide range of recombinant proteins.In this study,insect cell Sf9 were cultured in four kinds of bioreactor: spinner-flask,shake-flask,Bellocell and fermentation.Spinner-flask,shake-flask,Bellocell and fermentation were inoculated with 5×105/mL.Glucose,ammonia and lactate in the culture supernatant were measured.Cell density reached a maximum number of 5.5×106/mL and 7.3×106/mL when Sf9 were cultured in spinner-flask and shake-flask,which were 11 times and 14.6 times than the initial cell density.Cell density reached a maximum number of 8.01×106/mL and 1.52×107/mL when Sf9 were cultured in Bellocell and fermentor,which were 16.02 times and 30.4 times than the initial cell density.Insect cell Sf9 can be cultured in four different bioreactors for adhent culture and suspension culture with high cell density,especially in fermentor,and it makes Sf9 cells the suitable cells for generating recombinant protein in large scale.
【Fund】: 粤港关键领域重点突出项目(200749841104);; 广东省科技计划项目(20088030301349)
【CateGory Index】: Q813
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