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《Journal of Jinan University(Natural Science & Medicine Edition)》 2009-01
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Transcriptional responses of detoxification-related genes in the liver of Hypophthalmichthys molitrix and Ctenopharyngodon idellus intraperitoneally injected with microcystin-LR and lipopolysaccharide

WANG Lin,LIANG Xu-fang,LIU Xiu-xia,CHENG Wei-xuan,YU Ying(Department of Biotechnolgy,Jinan University,Guangzhou 510632,China)  
To study the transcriptional response of detoxification-related liver genes to microcystin-LR(MC-LR) and lipopolysaccharide(LPS) treatment,silver carp(Hypophthalmichthys molitrix) and grass carp(Ctenopharyngodon idellus) were respectively exposed to a single 50 μg kg-1 body weight(bwt) dose of pure MC-LR,a single 2 mg kg-1 bwt dose of LPS and a co-exposure MC-LR and LPS(50 μg kg-1 bwt + 2 mg kg-1 bwt),and were then sacrificed at 24 h post-exposure.Using beta-actin(β-ACT) as external control,the relative liver alpha-and rho-class soluble glutathione S-transferase(GSTA,GSTR),glutathione peroxidase(GPX),and uncoupling protein 2(UCP2) mRNA abundance of silver carp and grass carp were determined by real time-quantitative PCR.Although not significant,hepatic phase II xenobiotic metabolizing enzyme GSTA and GSTR in response to MC-LR still play a potential role for fish in MC-LR metabolism.The different induction might relate to the tolerance of the freshwater fishes to microcystins,toxin dose,as well as exposure time.The rather complex and insignificant response of GST gene to the effect of LPS exposure,might possibly be critical to the freshwater fishes to utilize toxic blue-green algae.In addition,GPX is essential to the detoxification of cyanotoxins by providing GSH for GST,and UCP2 also has an important role in inhibiting the excessive production of ROS to restrain fish hepatocytes apoptosis.
【Fund】: 国家自然科学基金项目(30670367);; 国家科技部863项目(2007AA09Z437);; 广东省科技计划项目(2007B020701002 2005B20301005)
【CateGory Index】: X174
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 WANG Lin,LIANG Xu-Fang,LIAO Wan-Qin,LEI La-Mei and HAN Bo-Ping (College of Life Science and Technology,Jinan University,Guangzhou 510632);MOLECULAR CLONING AND IN VIVO EXPRESSION ANALYSIS OF MICROCYSTIN DETOXIFICATION-RELATED GENES IN NILE TILAPIA(OREOCHROMIS NILOTICUS)[J];Acta Hydrobiologica Sinica;2007-06
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 Zou Wenwen Cao Yanqiu Gong Aijun University of Science and Technology Beijing Beijing 10083;Development of studies on microcystin in eutrophicated fresh water body in Beijing[J];Chemical Engineering & Equipment;2007-05
2 WANG Lin,LIANG Xu-fang,CHEN Xiao-yan,LI Guang-zhao,LIU Xiu-xia,HU Yong-le,YAO Yu(Department of Biotechnolgy,Jinan University,Guangzhou 510632,China);Transcriptional responses of detoxification-related genes to dietary Se and glutathione under the stress of microcystins-LR in tilapias[J];Journal of Jinan University(Natural Science & Medicine Edition);2010-01
3 WANG Lin,LIANG Xu-Fang,LIAO Wan-Qin,LEI La-Mei and HAN Bo-Ping (College of Life Science and Technology,Jinan University,Guangzhou 510632);MOLECULAR CLONING AND IN VIVO EXPRESSION ANALYSIS OF MICROCYSTIN DETOXIFICATION-RELATED GENES IN NILE TILAPIA(OREOCHROMIS NILOTICUS)[J];Acta Hydrobiologica Sinica;2007-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 He Jiawan; He Zhenrong; Guo Qionglin (Institute of Hydrobiology, Chinese Academy of Sciences. Wuhan,1430072);THE TOXICITY OF MICROCYSTIS AERUGINOSA TO FISHES AND DAPHNIA[J];Journal of Lake Science;1997-01
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