Experimental Investigation on the Molecularly Imprinted Polymer Receptor for Hydrochlorothiazide

DONG Yan1,QIU Yue-jin2,SUN Xian-xiang3(1.Department of Quality & Management,Hua Sheng Drug & Pharmaceutical limited Company,Changzhou 213022,China;2.Changzhou Bureau of Commodity Inspection & Quarantine on Entering and Exiting;3.Jiangsu Polytechnic University)  

A synthetic polymer selective to Hydrochlorothiazide was prepared by a non-covalently imprinted technique,using Hydrochlorothiazide as a template,methyl methacrylate as a functional monomer and tri-[hydroxymethyl]-propan-tri-acrylic acid as a cross-linker,and methanol as a porogenic solvent.After extraction of template,affinity of the polymer imprinted with Hydrochlorothiazide was examined in re-binding experiment.From the Scatchard analysis,binding site heterogeneity is obvious and two types of binding sites can be identified.The equilibrium dissociation constant Kd1,the apparent maximum amount Qmax1 and the binding constant Ka1 of the higher affinity binding sites can be calculated to be 57.0μmol/L,5.59μmol/g,and 1.75×104(mol/L)-1;while Kd2,Qmax2,and Ka2 of the lower affinity binding sites were calculated to be 3.96mmol/L,216.4μmol/g and 2.53×102(mol/L)-1,respectively.

【Key Words】： Hydrochlorothiazide molecularly imprinted polymer imprinting condition
【CateGory Index】： Q503

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