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《MYCOSYSTEMA》 1999-01
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MOLECULAR BIOLOGICAL CHARACTERIZATION OF LENTINULA EDODES MUTANT OBTAINED THROUGH SPACE MUTAGENESIS

JIA Jian-Hang; LI Chuan-You; JIN De-Min; LI Li-Yun;WANG Bin; WENG Man-Li(Institute of Genetics, Chinese Academy of Sciences, Beijing, 100101)(Biotechnique Center, Agricultural University of Hebei, Baoding, 071001)  
Through space mutagenesis, the phenotypic characters of Cr04, a Lentinula edodes strain, were improved obviously. In this study, DNA fingerprints of Cr04DZ and its control Cr04 were obtained with the methods of RAPD and AFLP.The polymorphism between Cr04 and Cr04DZ demonstrated in molecular level that the genetic material of Lentinula edodes has been varied after space mutagenesis. At the same time, RAPD and AFLP analysis indicated that the relative efficiency of AFLP for Lentinula edodes polymorphism detection was higher than that of RAPD. The reaction conditions for RAPD and AFLP were optimized. Through RAPD and AFLP analysis, 32 polymorphic products were detected between Cr04DZ and its control Cr04. 3 AFLP polymorphic products were cloned.
【Fund】: 863-2;;国家高技术航天领域项目!863-2-07-2;;北京市自然科学基金!1597200
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