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《MYCOSYSTEMA》 1999-01
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IDENTIFICATION AND PROPERTIES OF XANTHENE DYE DECOLORIZATION OF ASPERGILLUS WENTII WEHMER HD_1

LI Chan; BAI Jinghua; LIU Youqi (College of Life Science, Hubei University, Wuhan 430062)LI Lin(Department of Microbiology, Huazhong Agricultural University, Wuhan 430070)  
A dye-decolorizing fungus strain HD1 was isolated from soil and identified as Aspergillus wentii Wehmer. Strain HD1 was very effective to decolorize rose bengal, a kind of xanthene dye. The decolorizing rares were 93~99% and 89.3~98.8% respectively when temperature was 28~ 40℃ and pH 4. 0~ 8.0. The optimum temperature and pH of decolorization were 33℃ and 6.0 respectively. Media, carbon sources, nitrogen sources and inoculating amount could affect the decolorizing rates.The decolorizase was constitutive one and mainly intracellular. The ratio of intracellular or extracellular decolorase could be changed and the synthesis of intracellular enzymes could be stimulated when rose bengal was added to the medium.The rose bengal degraded products were determined by UV-visible spectrophotometer.The absorption peak in 544. 8nm was almost completely disappeared, while the absorption in UV district was increased, disappear or diminished.
【Fund】: 黑龙江省科委青年计划项目;;湖北大学校长基金
【CateGory Index】: Q939.56
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