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LI Gang;LI Baojian (Biotechnology Research Center, Zhongshan University, Guangzhou 510275)  
The conditions of separation and regeneration of Ganoderma tsugae protoplast were studied in detail and systematically for the first time. Results show that the highest production rate of protoplast can be obtained by using compound enzyme composed of 0.6mol / L mannitol, 2% lywallzyme and 0.5% driselase and digesting for 3 hours at the condition of 30℃ and pH value 6.0. However, taking account of the regeneration of protoplast, it is most suitable to digest for 2.5 hours.The protoplast produced by 2.5-hour enzymolysis was purified and regenerated through double layers plate method in cellobiose medium with 0.6mol / L mannitol used for stabilizing osmotic pressure. The highest regeneration rate was obtained. This study has laid the foundation of protoplast fusion and transgenic research of Ganoderma in the future.
【Fund】: 广东省自然科学基金!940127
【CateGory Index】: Q932
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