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《MYCOSYSTEMA》 1999-01
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CONSTRUCTION OF THE THERMOTOLERANT YEASTS BY PROTOPLAST FUSION BETWEEN KLUYVEROMYCES AND SACCHAROMYCES

WEN Tieqiao (Institute of Life Science, Hubei University, Wuhan 430062)ZHAO Xuehui (Department of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070)  
A thermotolerant yeast strain was constructed by protoplast fusion between Saccharomyces cerevisiae and respiratory defective Kluyveromyces sp.inactivated with iodoacetate. The fusant AY006 has a high ethanol yield (up tO 8.7%),using glucose as substrate at 45℃. Reversion inhibition of high density in protoplast was extensively decreased by deleting mitochondrion and adding chloromycetin.Meanwhile, colony morphology, isozymic feature and high temperature fermentation were studied. The result shows that the fusant greatly expressed fine properties of parent strains in thermotolerance and ethanol yield.
【Fund】: 农业部八五攻关!85-农-05-01-05
【CateGory Index】: Q933
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