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《Mycosystema》 2017-01
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Effects of antinocieptive compound from Ophiocordyceps sobolifera on the transcriptome and Adora1 etc. pain-related genes in gouty rats

ZHU Wei-Jian;CHAI Yi-Qiu;JIN Yi-Wei;LI Xiao-La;YU Xiao-Ping;Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University;Zhejiang Institute of Subtropical Crops, Key Laboratory of Entomogenous Fungus Resources Research and Development of Wenzhou;  
N~6-(2-hydroxyethyl)-adenosine(HEA) separated and purified from the artificial cultivation of Ophiocordyceps sobolifera was confirmed to be responsible for analgesia of acetic acid-stimulated mice. The m RNA expression of synovium from gouty rats treated with or without HEA was compared by transcriptome sequence and 932 differential expressed genes(DEGs) were obtained. Comparison of these 932 DEGs with the pain gene database, where the genes were changed during pain, 57 genes were supposed to be highly potential to participate in the regulation of pain. According to related reports, 12 antinocieptive genes were obtained including up-regulated adenosine A1 receptor(A1R, Adora1) and A2 A receptor(A2AR, Adora2a). The result of Western blot showed that A1 R and A2 AR, the regulated target of pain, were markedly increased and decreased respectively in HEA group(7.5mg/kg) as compared with model group, and the effect of HEA on A1 R was reversed by the administration of selective A1 R antagonist DPCPX. These result demonstrated that HEA took the responsibility for the analgesia of gouty rats, and HEA may exert its antinociception by the activation of A1 R, down-regulated A2 AR and regulation of other pain-related genes.
【Fund】: 温州市种子种苗创新项目(N20150010);; 温州市四新项目(F-DY201509170025);; 温州市公益性科技项目(N20140037)~~
【CateGory Index】: R285.5
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