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《Mycosystema》 2017-01
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Effects of light and pH on the antioxidant activities of medicinal fungus Trichaptum biforme during liquid cultivation

YANG Xiao-Po;ZHENG Fei;LIU Tie-Zhi;MENG Ge;SI Jing;College of Life Sciences, Chifeng University;Research Center for Philosophy of Science and Technology, Shanxi University;Institute of Microbiology, Beijing Forestry University;  
Based on the tested indicators of polyphenol contents, total antioxidant capacity(T-AOC), 1,1-diphenyl-2-picrylhydrazyl(DPPH) radicals scavenging activities, and superoxide dismutase(SOD) activities, effects of different light and pH conditions on the antioxidant activities of medicinal fungus Trichaptum biforme during liquid cultivation were explored. The results demonstrated that T. biforme had antioxidant activities during submerged culture. Light treatment to a certain extent could enhance secretion capacity of secondary metabolites and antioxidant activities of the fungus. After being illuminated for 14 d, the strain was shown to exert the highest capacities for secreting polyphenol and T-AOC, with the corresponding yields of 1.85mg/m L and 3.99U/m L on the 12 th and 10 th days. Higher DPPH radical scavenging efficiency and SOD activities were observed under alternation condition of dark for 1d and light for 1d, reaching their peaks of 87.44% and 0.63U/m L on the 2nd day. Effects of different pH conditions on the antioxidant activities of T. biforme during liquid cultivation were greater. When the pH was 9.0, the fungus exhibited better capacities of secreting polyphenol, T-AOC, and SOD, and performed remarkable scavenging activities against DPPH radicals, with respective values of 2.92mg/m L, 5.22U/m L, 0.70U/m L, and 95.07% on the 2nd, 10 th, 2nd, and 2nd days. T. biforme showed the highest antioxidant activities at pH 9.0, and secondly by pH 3.0, 5.0, and 7.0, suggesting that relatively alkali or acidic conditions could stimulate the medicinal fungus T. biforme to secret more secondary metabolites to resist the oxidative stress from microenvironment.
【Fund】: 中央高校基本科研业务费专项资金(2016ZCQ04)~~
【CateGory Index】: S567.39
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