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《Mycosystema》 2017-01
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Sterone content determination and fingerprint analysis of Polyporus umbellatus sclerotia using high performance liquid chromatography (HPLC)

CHEN Xiao-Mei;ZHOU Wei-Wei;WANG Chun-Lan;YANG Jun-Shan;GUO Shun-Xing;Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & Peking Union Medical College;  
Thirty five Polyporus umbellatus sclerotium samples were collected from 12 provinces of China. A HPLC method was developed for simultaneous quantitative determination of five sterone compounds in the samples. HPLC fingerprint was used to analyze the samples, and the similarity of fingerprint was calculated by the similarity evaluation system for chromatographic fingerprint of Chinese traditional medicine. Quantitative analysis showed that a total of 28 samples could obtain content determination results, and polyporusterone A and B accounted for more than 70% of the total sterone compounds in 25 of them. Correlation analysis showed a great significant positive correlation between the content of polyporusterone A and B(r=0.955, P0.01). The samples from Shaanxi Province had the highest levels of average total sterone content and polyporusterone A and B relative content, being 182.70μg/g(C.V=82.4%) and 92.3%(C.V=3.1%) respectively. The similarity between the fingerprints of the 35 samples was 0.412–0.943. A total of eight common peaks could be found and three of them could be identified. The similarity between the fingerprints of the 11 samples from Shaanxi Province was 0.812–0.989. A total of 17 common peaks could be found and nine of them could be identified. The results suggested that polyporusterone A and B were the main sterone components in P. umbellatus sclerotia, and played an important role in the quality control of medicinal materials. It was also proved that the quality of P. umbellatus sclerotia from Shaanxi Province was good and stable.
【Fund】: 国家重点基础研究发展计划(2014CB138304)~~
【CateGory Index】: S567.3
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