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《Mycosystema》 2018-01
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The endophytic fungal community composition of Gymnadenia conopsea in Beijing

CHEN Yan-Hong;XING Xiao-Ke;GUO Shun-Xing;Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences;  
Gymnadenia conopsea is a terrestrial orchid,a kind of traditional Chinese herbal medicine.Based on Illumina Mi Seq high-throughput sequencing technology targeting ITS1 region,endophytic fungal communities associated with G.conopsea growing in Beijing were analyzed.A total of 50 420 effective sequences were selected and classified into 209 operational taxonomic units(OTUs),belonging to 54 families,28 orders,9 classes,and 3 phyla.Nine classes correspond to Pezizomycetes(3OTUs),Agaricomycetes(21 OTUs),Leotiomycetes(18 OTUs),Sordariomycetes(51 OTUs),Zygomycetes(18 OTUs),Tremellomycetes(19 OTUs),Eurotiomycetes(22 OTUs),Saccharomycetes(8 OTUs),and Dothideomycetes(14 OTUs).The endophytic fungi belonging to Cantharellales(38.62%)and Helotiales(24.64%)were found to be dominant in the roots of G.conopsea from Baihua Mountain,and species of Pezizales(66.19%)and Cantharellales(29.75%)were dominant in Song Mountain.These results laid a foundation for understanding the endophytic fungal community composition of G.conopsea in Beijing and applying endophytic fungi to artificial breeding of the orchid.
【Fund】: 中国医学科学院医学与健康科技创新工程-重大协同创新项目(2016-I2M-2-002)~~
【CateGory Index】: Q93;S567.239
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