An insight into the high-efficient gene-editing techniques in Aspergillus
ZHANG Chi;GU Hui-Yu;LU Ling;Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University;
Aspergillus is one of the most common airborne fungal genus in which Aspergillus niger plays important roles in production of food additives while a human fungal pathogen Aspergillus fumigatus is a fatal-threat species, especially for immunocompromised patients worldwide. Therefore, establishing an efficient gene-editing system will give insight into the dynamic physiological process for these fungal species. In this review, various gene-editing techniques including traditional homologous integration using flanking regions and advanced CRISPR-Cas9(clustered regulatory interspaced short palindromic repeats-associated protein 9) gene-editing techniques used in different species of Aspergillus are summarized. This information may provide useful clues to further conduct efficient gene editing in other un-explored Aspergillus species.