Preparation of Monodispersed Molecularly Imprinted Polymeric Microspheres by One-step Swelling Polymerization

YANG Ming,HUAI Lu-feng,ZHAO Tao,LIU Wei,ZHAO Jing-guo(Department of Chemical and Environmental Engineering,Wuhan Polytechnic University,Wuhan 430023,Hubei,China)  

Molecularly imprinted polymeric microspheres(MIPMs) were prepared by one-step swelling polymerization method in aqueous system using micron-polystyrene microspheres prepared by emulsifer-free polymerization as seeds and using N-methylasparagic acid(NMA) as cyclostyle molecules.Good monodispersed MIPMs were prepared with particle size in the range of 2～3 μm.Scatchard analysis suggested the MIPMs recognizing NMA with two classes of binding sites.The calculated dissociation constant KD1 and apparent maximum number Bmax1 of binding sites with high affinity were 0.223 mmol/L and 2.12 μmol/g respectively,while KD2 and Bmax2 of binding sites with low affinity were 5.34 mmol/L and 36.42 μmol/g.

【Key Words】： molecularly imprinted polymeric microspheres single-step swelling polymerization monodispersity binding sites
【CateGory Index】： O631.5

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