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Study on Tissue Culture of Moringa Lam

LUO Yunxia1,LU Bin2,SHI Zhuogong1(1.Southwest Forestry College,Kunming Yunnan 650224,China; 2.Yunnan Academy of Forestry,Kunming Yunnan 650204,China)  
The sterilized seeds of Moringa oleifera Lam started to germinate after they were striped and growned on MS media for 10 days.The germinate rate was up to 79%.The suitable disinfection way of M.oleifera's seeds was that soaked by H2O2 for 6~8h,cleaned by water,then sterilized with 0.1% HgCl2 25~40min.Under the common temperature,the sprig of germinated seeds could produce bud cluster and proliferation on the media of MS+6-BA 0.1 mg/L+KT 0.5 mg/L+cane suger 30%+agar 6g/L,and the proliferation rate could be more than 6 times.The media for multiplication was MS+6-BA0.6mg/L+NAA0.1mg/L+cane suger30%+agar 6 g/L,and the proliferation rate could be 5.8;The suitable media for rooting was 1/2 MS+IBA 0.05~0.5+Suger 15%,and the rooting rate could be over 80%,the best was up to 98% when IBA was 0.1 mg/L.The survival ratio was 80% when grown in nursery.The best pH range is 5.8~6.2.
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