Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Agriculturae Universitatis Jiangxiensis》 2017-01
Add to Favorite Get Latest Update

Construction of atp9 Gene RNA Interference Expression Vector of Tobacco and Its Genetic Transformation

XIE Li-juan;TAO Yao;ZHONG Si-rong;WU Ling-min;NIE Ya-ping;ZHOU Wei;LIU Dong;WANG Jian-ge;LIU Qi-yuan;Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Key Laboratory of Crop Physiology,Ecology and Genetic Breeding of Jiangxi Province/College of Agronomy,Jiangxi Agricultural University;Nanchang Career Academy;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,College of Bio-safety Science & Technology,Hunan Agricultural University;College of Landscape Architecture and Art,Jiangxi Agricultural University;  
Atp9 gene is a tobacco male sterility related gene.In order to study the function of atp9 gene and its effect on the formation of male sterility in tobacco,according to the sequence of atp9 gene,a pair of specific primers was designed to amplify sense and antisense interference fragment of atp9 gene( 239 bp),which was inserted into the right and left sides of linker respectively,and the RNA interference vector was inserted into the plant expression vector pCAMBIA1301,then the pCAMBIA1301-atp9-RNAi was successfully constructed.The pCAMBIA1301-atp9-RNAi was transferred into tobacco male-fertile varieties Zhongyan 90 with agrobacterium mediated method,the results showed that the p CAMBIA1301-atp9-RNAi interference vector had been successfully transferred to tobacco male-fertile varieties Zhongyan90. Finally,41 transgenic pCAMBIA1301-atp9-RNAi tobacco plants were obtained,and the molecular detection of 20 plants was positive,its conversion rate was 48.8%.So,the study laid a foundation for the next step to verify the function of atp9 and its effect on the formation of male sterility in tobacco.
【Fund】: 国家自然科学基金项目(31260350 31301388);; 江西省教育厅科技计划项目(GJJ13275)~~
【CateGory Index】: S572;Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 XIAO Hai-jun;ZOU Chao;CHEN Jun-hui;CHEN Li-yuan;Institute of Entomology,Jiangxi Agricultural University;;The Screening Methods for Differential Expression Genes Related to Insect Diapause[J];江西农业大学学报;2014-06
2 YUAN Xiu-yun;LIANG Fang;JIANG Su-hua;WANG Mo-fei;LIU Jia;CUI Bo;Institute of Bioengineering,Zhengzhou Normal University;College of Life Science,Henan Agricultural University;;Cloning,Expression and Vector Construction of A PEBP Family Gene in Oncidium[J];江西农业大学学报;2014-06
3 WANG Zong-yao1,WU Ye-bao1,OUYANG Xiang-yang1,YU Ji-sheng1,MENG Jian2(1.Ji’an Academy of Agricultural Sciences,Ji’an 343103,China;2.Hebei Agricultural Technology Extension General Station,Shijiazhuang 050000,China);Progress on Application of RNA Interference in Soybean[J];大豆科学;2013-04
4 Yang Fei, Zhu Rui, Lin Na, Liu Feihu ( Laboratory of Plant Improvement and Utilization, Yunnan University , Kunming 650091);Construction of Male Sterility Related Genes atp6 and atp9 RNAi Vectors in Ramie[J];中国农学通报;2013-21
5 CHEN Xingjiang,CAI Liuti,and ZHENG Shaoqing Guizhou Tobacco Science Institute,Guiyang 550081,China;Tobacco Plant Axillary Bud Related Gene Cloning and RNAi Expression Vector Constructing and Transforming[J];烟草科技;2013-03
6 ZHANG Zhi1,2,LIANG Yi1,DING Hai-feng1,ZHANG Hong-wei1(1.Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in North China,Beijing Jingyan Yinong Sci-Tech Developent Center,Beijing 100097;2.Shanxi Agriculture University,Taigu,Shanxi 030800);Study on Cloning and Expression of atp9 Gene in Carrot and Its Relationship to Cytoplasmic Male Sterility[J];北方园艺;2012-24
7 WANG Weifeng,TAI Shuaishuai,WANG Lu,LIU Guanshan,LI Fengxia,GAO Xiaoming,SUN Yuhe(Key Laboratory of Tobacco Quality Control,Ministry of Agriculture,Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China);Construction of RNAi Vector of NtLS Gene and its Transformation in Tobacco[J];中国烟草科学;2011-04
8 ZHANG Gai-yun1,XIONG Teng-fei2,MA You-zhi3,GUO Jia-ming2,CHEN Xue-ping21 Key Laboratory of Marine Bio-resources Sustainable Utilization,South China Sea Institute of Oceanology,Chinese Academy of Science,164 West Xingang Road,Guangzhou 510301,China;2 Department of Chemistry,University of Science and Technology of China,96 Jinzhai road,Hefei 230026,China;3 Institute of Crop Science,Chinese Academy of Agricultural Sciences,12 Zhongguancun South Street,Beijing 100081,China;The construction of tobacco CN gene RNAi vector and its function analysis[J];中国烟草学报;2010-04
9 ZHANG Fuyun1,LI Yan1,LI Yunbing1,ZHANG Ying1,ZHAO Xiaoming2 and DU Yuguang2(1.Dalian Fisheries University,Dalian Liaoning 116023,China;2.Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Liaoning Provincial Key Laboratory of Carbohydrate,Dalian Liaoning 116023,China);Construction of RNAi Expression Vector of NtSKP1 and Transformation in Tobacco[J];西北农业学报;2010-08
10 LIU Ai-ling1 ZOU Jie1 WANG Wen-fang1 ZHOU Xiao-yun2 ZHANG Xian-wen1 CHEN Xin-bo1,2(1.College of Bioscience and Technology of Hunan Agricultural University,Changsha,Hunan 410128;2.Crop Gene Engineering Key Laboratory of Hunan Province,Changsha,Hunan 410128);CONSTRUCTION OF RNAi EXPRESSION VECTOR AND TRANSFORMATION OF OSHSFA7 GENE IN Oryza sativa L.[J];核农学报;2010-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Shanshan;YANG Jun;WANG Zhong;LIN Fucheng;PAN Ting;WU Mingzhu;ZHANG Jianfeng;CAO Peijian;XIE Xiaodong;Zhengzhou Tobacco Research Institute of CNTC;Zhejiang University;Zhengzhou University;;Targeted mutagenesis of NtLS gene using CRISPR/Cas9 system[J];烟草科技;2018-02
2 CHEN Lili;XIONG Anping;SHI Weinan;ZHOU Jialin;ZHANG Ye;SHENG Yunyan;Agriculture College, Heilongjiang Bayi Agricultural University;;Performance Analysis of Male Sterility Line ms2 in Melon[J];蔬菜;2017-09
3 Zhong Sirong;Xie Lijuan;Tao Yao;Wu Lingmin;Nie Yaping;Zhou Wei;Wang Jiange;Liu Qiyuan;Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Key Laboratory of Crop Physiology, Ecology and Genetic Breeding of Jiangxi Province, College of Agronomy, Jiangxi Agricultural University;Nanchang Career Academy;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University;College of Landscape Architecture and Art, Jiangxi Agricultural University;;Construction of orf25 Gene RNA Interference Expression Vector and Transformation of Tobacco[J];分子植物育种;2017-05
4 Zhang Ya-nan;Lin Yin-ding;Meng Li;Mao Pei-chun;Tian Xiao-xia;Guo Qiang;Shanxi Agricultural University;Beijing Research and Development Center for Grasses and Environment,Beijing Academy of Agriculture and Forestry Science;;Construction of RNAi expression vector of heavy metal ATPases gene HMA2 and genetic transformation in Iris lactea[J];草业科学;2017-05
5 XIE Li-juan;TAO Yao;ZHONG Si-rong;WU Ling-min;NIE Ya-ping;ZHOU Wei;LIU Dong;WANG Jian-ge;LIU Qi-yuan;Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Key Laboratory of Crop Physiology,Ecology and Genetic Breeding of Jiangxi Province/College of Agronomy,Jiangxi Agricultural University;Nanchang Career Academy;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,College of Bio-safety Science & Technology,Hunan Agricultural University;College of Landscape Architecture and Art,Jiangxi Agricultural University;;Construction of atp9 Gene RNA Interference Expression Vector of Tobacco and Its Genetic Transformation[J];江西农业大学学报;2017-01
6 XIE Lijuan;RAO Wenping;WU Lingmin;TAO Yao;NIE Yaping;ZHOU Wei;LIU Qiyuan;Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Key Laboratory of Crop Physiology,Ecology and Genetic Breeding of Jiangxi Province , College of Agronomy,Jiangxi Agricultural University;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,College of Bio-safety Science and Technology,Hunan Agricultural University;;Advancements of RNA Interference Technique and Its Application in Tobacco[J];云南农业大学学报(自然科学);2016-06
7 Tao Yao;Wang Yu;Wu Linmin;Xie Lijuan;Nie Yaping;Zhong Sirong;Zhou Wei;Wang Jiange;Liu Qiyuan;Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Key Laboratory of Crop Physiology, Ecology and Genetic Breeding of Jiangxi Province, College of Agronomy, Jiangxi Agricultural University;Agricultural committee of Guizhou province Qianxinanzhou;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Bio-safety Science and Technology, Hunan Agricultural University;College of Landscape Architecture and Art, Jiangxi Agricultural University;;PCR Amplification and Sequence Analysis of CMS orfB Gene in Tobacco[J];基因组学与应用生物学;2016-06
8 CHEN Ya-qiong;SUN Ting-ting;CHEN Lei;DING An-ming;CHEN Huan;SUN Yu-he;Key Laboratory for Tobacco Gene Resources,Tobacco Research Institute of Chinese Academy of Agricultural Sciences;Graduate School of Chinese Academy of Agricultural Sciences;;Molecular Cloning and Expression Analysis of BRANCHED1-Like Gene in Common Tobacco[J];植物遗传资源学报;2015-06
9 PAN Lidan;YAO Dan;WANG Piwu;LIU Tingting;ZHENG Chengzhong;ZHAO Cuilan;College of Agronomy,Jilin Agricultural University;College of Life Science,Jilin Agricultural University;;Cloning of Gm FBDG01 Gene in Soybean Flowering Bud and Construction of RNA Interference Expression Vector[J];吉林农业大学学报;2015-05
10 Duan Lijie;Wang Pei;Chen Mengci;Wang Suomin;State Key Laboratory of Grassland Agro-ecosystems,College of Pastoral Agriculture Science and Technology,Lanzhou University;;Cloning Outward-Rectifying Potassium Channel PtSKOR Gene and Constructing Its RNAi Vector in Halophyte Puccinellia tenuiflora[J];分子植物育种;2015-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LI Xia,DAI Jia-le,CHEN Zi-qi,FU Yong-ping,MA Jian,QU Jing,WANG Pi-wu(Biotechnology Center,Jilin Agricultural University,Changchun,Jilin130118,China);Construction of the ihp-RNAi expression vector of soybean β-conglycininα′-subunits gene[J];西北农林科技大学学报(自然科学版);2012-10
2 CHEN Zi-qi,LI Xia,WANG Pi-wu,FU Yong-ping,LI Lu-hua,MA Jian,QU Jing(Biotechnology Center,Jilin Agricultural University,Changchun 130018,Jilin,China);Using RNA Interference Technology to Improve Soybean Fat Content[J];大豆科学;2012-04
3 CHEN Hao,YANG Jie,CHENG Wei-ning,WU Jun-xiang(State Key Laboratory of Crop Stress Biology for Arid Areas/Key Laboratory of Applied Entomology,Northwest A&F University, Yangling,Shaanxi 712100,China);Construction and analysis of suppression subtractive hybridization cDNA library about the diapause and non-diapause larvae of Grapholita molesta(Busck)[J];西北农林科技大学学报(自然科学版);2012-09
4 LI Dongmei,MENG Fanli,WANG Zhikun,ZANG Zhenyuan,SUN Jing,LI Wenbin(Key Laboratory of Soybean Biology,Ministry of Education,School of Agriculture,Northeast Agricultural University,Harbin 150030,China);Cloning and characterization of 28SrDNA gene from soybean pod borer(Leguminivora glycinivorella Matsumurav)[J];东北农业大学学报;2012-07
5 ZHANG Shuai,CUI Jin-Jie*,WANG Chun-Yi,LUO Jun-Yu,Lü Li-Min(State Key Laboratory of Cotton Biology,Cotton Research Institute,Chinese Academy of Agricultural Sciences,Anyang,Henan 455000,China);cDNA library construction and EST analysis of larval salivary glands of Helicoverpa armigera(Lepidoptera:Noctuidae)[J];昆虫学报;2012-06
6 ZHENG Bai-ling,ZHANG Hong-li#,ZHOU Qian-jin,CHEN Xue-qiu,DU Ai-fang*(Key Laboratory of Animal Epidemic Etiology & Immunological Prevention of Ministry of Agriculture,Institute of Preventive Veterinary Medicine,College of Animal Sciences,Zhejiang University,Hangzhou 310058,China);Screening and identification of diapause-related genes from Haemonchus contortus by mRNA differential display technique[J];中国兽医学报;2012-05
7 ZHANG Zan1 LIU Jin-Ding1,2 HUANG Shui-Qing2 LI Fei1** (1. Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China; 2. College of Information Science and Technology, Nanjing Agricultural University, Nanjing 210095, China);Application of bioinformatics in entomology[J];应用昆虫学报;2012-01
8 Wang Xinduo,Li Li,Wang Xifeng (State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing100193,China);Application and biosafety of transgenic antiviral crops based on RNA interference[J];植物保护;2011-06
9 YE Gong-Yin FANG Qi(Key Laboratory of Agricultural Entomology of Ministry of Agriculture,Institute of Insect Sciences,Zhejiang University,Hangzhou 310029,China);Entomological research in the genomic age[J];应用昆虫学报;2011-06
10 XU Zong-hong1,2,3,HAO Qing-nan1,2,CHEN Li-miao1,2,SUN Dian-chen1,2,TIAN Xing-xing1,2,SHAN Zhi-hui1,2(1.Oil Crops Research Institute,Chinese Academy of Agriculture Sciences,Wuhan 430062,China;2.Key Laboratory of Oil Crop Biology,Ministry of Agriculture,Wuhan 430062,China;3.Yangtze University,Jingzhou 434023,China);Plant Expression Construct Based on RNAi for Soybean Mosaic Virus[J];华北农学报;2010-S2
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved