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《Acta Agriculturae Universitatis Jiangxiensis》 2017-01
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Construction of SSR Fingerprint on 33 Ancient Chestnut Trees

LIU Guo-bin;CAO Jun;LAN Yan-ping;WANG Jin-bao;Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences;Beijing Huairou Chestnut Experiment Station;  
For the purpose of genetic identification and scientific protection of ancient chestnut trees,SSR technology was used to establish fingerprinting for 33 ancient chestnut trees,which were planted during Ming and Qing dynasties.The polymorphism of 33 ancient chestnut trees was analyzed with capillary electrophoresis detection method with fluorescent SSRs markers.Totally,61 polymorphic alleles were revealed by 13 SSR primer pairs,with an average of 4.69 for each primer pair.The polymorphism information content values of the primer pairs ranged from 0.060 1 to 0.800 9,with an average of 0.410 3. A strategy of combining primer pair with distinct alleles for fingerprint construction was developed and applied to the 33 ancient chestnut trees.The present results provide valuable information for the protection,germplasm classification and identification,and breeding of chestnut.
【Fund】: 中央财政林业科技推广示范资金项目(京园绿推[2014]05号)~~
【CateGory Index】: S664.2
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