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《科学通报(英文版)》 2003-01
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Nitric oxide produced by cumulus cells stimulates maturation of mouse oocytes

BU Shumin, XIA Guoliang, XIE Huirong & GUO Yong College of Biological Science, China Agricultural University, Beijing 100094, China Correspondence should be addressed to Xia Guoliang (e-mail: glxia@ mail.cau. edu.cn)  
The effect of sodium nitroprusside (SNP), a donor of nitric oxide, on meiotic maturation of mouse oo-cytes was studied by injecting Nw-nitro-L-arginine methyl ester (L-NAME) intra-peritoneal (ip), a nitric oxide synthase inhibitor, or culturing oocytes in the medium supplemented with L-NAME or hypoxanthine (HX) to arrest the sponta-neous oocyte maturation in vitro. The results showed that the inhibitory effect of L-NAME by injecting 10 mg/kg ip on extrusion of the first polar body only could be reversed by injecting 2.5 mg/kg SNP with L-NAME simultaneously (P 0.05). Half an hour later ten mice died when given 10 mg/kg SNP ip. The treatment of some concentrations of SNP (10-7,10-6,10-5mol/L) significantly stimulated meiotic matu-ration to metaphase Ⅱ stages in cumulus enclosed oocytes in the presence of HX. However, other concentrations of SNP (10-8, 10-4, 10-3 mol/L) had no effect on HX-arrested oocyte meiotic maturation. The optimal concentration of SNP on CEOs had no effect on DOs. The dose of 10-3 mol/LL-NAME demonstrated a significant suppression in formation of PB1, but not in GVBD. This inhibition was reversed by the addi-tion of SNP. These results indicated that the physiological levels of NO produced by cumulus cells could stimulate mei-otic maturation of mouse oocytes both in vivo and in vitro.
【Fund】: This work was supported by the National Out-standing Youth Foundation (Grant No. 30025032) and the Natural Sci-ence Foundation of Beijing (Grant No. 6992019).
【CateGory Index】: Q492.6
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