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《科学通报(英文版)》 2003-01
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Calcium-releasing activity induced by nuclei of mouse fertilized early embryos

LUO Xiaoyan, HUANG Xiuying & SUN Fangzhen Key Laboratory of Molecular Developmental Biology, Institute of Ge-netics and Developmental Biology, Chinese Academy of Sciences, Bei-jing 100080, China Correspondence should be addressed to Sun Fangzhen or Huang Xiuying (e-mail: sunfangzhen@sina.com or suehxy@sina.com)  
At fertilization, repetitive transient rises of intracellular calcium concentration occur in all mammals studied so far. It has been shown that calcium rises could be induced when mouse fertilized 1-, 2-cell nuclei were trans-planted into unfertilized eggs and that the reconstituted em-bryo could be activated. However, whether the capability of inducing calcium rises occurs in all stages of mammalian embryos remains unknown. In this study, by using the nu-clear transplantation technique and measurement of intra-cellular calcium rises in living cells, we showed that only the nuclei from mouse fertilized 1-cell and 2-cell embryos, nei-ther the nuclei from 4-, 8-cell and ethanol activated parthe-nogenetic embryos nor 2 or 3 nuclei of electrofused 4-cell stage syncytium, have calcium-releasing activity when they were transferred into unfertilized mature oocytes. Our results indicate that the calcium-releasing activity in nuclei of 1-, 2-cell embryos is produced during fertilization and exists at the special stage of fertilized early embryos. These suggested that the capacity of inducing calcium release ac-tivity in fertilized early embryos is important for normal embryonic development.
【Fund】: This work was supported by the State Key Basic Research Program of China (Grant No. G1999055902) and the Funds of the Chinese Academy of Sciences (Grant Nos. KJ951-B1-607 and STZ-00-15).
【CateGory Index】: Q492.6
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