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《科学通报(英文版)》 2003-03
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Effects of calmodulin on DNA-binding activity of heat shock transcription factor in vitro

LI Bing1,2, LIU Hongtao1,2, MU Ruiling1,2, SUN Daye2& ZHOU Rengang1 1. Institute of Agro-Physics, Physiology and Biochemistry, HebeiAcademy of Agricultural and Forestry Sciences, Shijiazhuang 050051,China; 2. Institute of Molecular Cell Biology, Hebei Normal University, Shijiazhuang 050016, China  
The DNA-binding activity of heat shocktranscription factor (HSF) was induced by heat shock (HS) of a whole cell extract. Addition of antiserum, specific toCaM, to a whole cell extract reduced bind of the HSF to the heat shock element (HSE) with maize, and the re-addition of CaM to the sample restored the activity of the HSF forbinding to HSE. In addition, DNA-binding activity of the HSF was also induced by directly adding CaM to a whole cell extract at non-HS temperature with maize. Similar resultswere obtained with wheat and tomato. Our observationprovide the first example of the involvement of CaM inregulation of the DNA-binding activity of the HSF.
【CateGory Index】: Q523
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Chinese Journal Full-text Database 6 Hits
1 FENG Lei et al(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua,Zhejiang321004);Heat Shock Transcription Factors on Defense Responses in Plant[J];Journal of Anhui Agricultural Sciences;2011-04
2 LI Bing,LIU Hong tao,ZHOU Ren gang (1 Institute of Agriphysics,Physiology and Biochemistry,Hebei Academy of Agricultural and Forestry sciences, Shijiazhuang 050051,China;2 College of Life Science,Hebei Normal University,Shijiazhuang 050016,China);Progress on Research of Heat Shock Transcription Factors[J];Acta Agriculturae Boreall—sinica;2000-S1
3 ZHA Zhong-ping,WAN Bing-liang(Institute of Food Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China);Advance in the Research of Heat Stress Transcription Factors in Plants[J];Hubei Agricultural Sciences;2009-09
4 GUO Li-hong1,WANG Ding-kang1,YUAN Yan1,LIU Kai-qing1,ZHANG Le-min2 (1.Life Science and Technology Department,Kunming University,Yunnan Kunming 650031,China;2.School of Life Sciences,Yunnan University,Yunnan Kunming 650092,China);Expression and Purification of Heat Shock Factor HsfA1a Polypeptide in Arabidopsis thaliana[J];Journal of Kunming University;2009-03
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【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 WANG Qing-Lin1,DONG Yun-Wei2,DONG Shuang-Lin1,WANG Fang1,SONG Wen-Hua1,YU Shan-Shan1(1.The Key Laboratory of Mariculture,Ministry of Education,College of Fisheries,Ocean University of China,Qingdao 266003,China;2.College of Oceanography,Xiamen University,Xiamen 361005,China);Effects of Heat-Shock in Pelagic Stages on Survival and Hsp70 Expression of Juvenile Sea Cucumber,Apostichopus japonicus Selenka[J];Periodical of Ocean University of China;2011-03
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