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《科学通报(英文版)》 2003-03
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B→O blood conversion

ZHANG Yangpei1, YANG Jun1, GAO Xin1, JIA Yanjun1, JI Shouping1, GONG Feng1, LIU Zepeng1, REN Huiming1, LI Subo1, LAN Jiongcai2 & CAO Qiong2 1. Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China; 2. South Hospital, The First Military Medical University, Guangzhou 510515, China  
To maintain a constant supply of universal blood type, or group O red blood cells, has benefit in specialized transfusion condition. In this study, a-galactosidase cDNA was cloned from Catimor coffee bean grown in Hainan island, China, by the RT-PCR method. We have constructed a vector for a-galactosidase cDNA expression and transferred a-galactosidase cDNA into Pichia pastoris GS115 cells by elec-troporation. The recombinant a-galactosidase (r-a-GalE) was purified by cation ion exchange chromatography. After studying the biochemical characters of r-a-GalE, we have succeeded in converting human erythrocytes from group B to group O. The animal experiment showed that transfusion of enzymetically converted group O red blood cells (ECORBC) was perfectly safe.
【CateGory Index】: R457
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Chinese Journal Full-text Database 1 Hits
1 ZHANG Yang-Pei JI Shou-Ping YANG Jun GONG Feng YOU Ying (Beijing Institute of Blood Transfusion Medicine Beijing 100850);Conversion of blood groups[J];Journal of Experimental Hematology;1998-02
Chinese Journal Full-text Database 10 Hits
1 CHEN Fang-xiang,HE Jing,LIU Lin,et al. (Department of Blood Transfusion,Daping Hospital,Third Military Medical University,Chongqing 400042,China);Analysis of blood transfusion in 688 traumatic cases[J];Journal of Traumatic Surgery;2003-04
2 LIU Ze_Peng 1,CAO Qiong 2, GAO Xin 1, YANG Jun 1, LI Su_Bo 1, LAN Jiong_Cai 2, ZHANG Yang_Pei 1 (1.Beijing Institute of Blood Transfusion Medicine ,Beijing 100850,China;2.Nanfang Hospital,the First Military Medical University, Guangzhou 510515,China);B→O Blood type conversion in animal experiments:enzymatic digestion of human-like B antigens on rhesus monkey red blood cells by α-galactosidase[J];Bulletin of The Academy of Military(Medical Sciences);2003-02
3 LIU Ze-Peng1, ZHANG Cheng-Lin2, LU Yan-Ping2, YANG Ming-Hai2, ZHANG Jin-Guo2, GAO Xin1, JIA Yan-Jun1, GAO Hong-Wei1, BAO Guo-Qiang1, LI Su-Bo1, ZHANG Yang-Pei 1* (1.Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China; 2.Beijing Zoo, Beijing 100044, China);Study of gibbon experiment on B to O blood type conversion[J];Bulletin of The Academy of Military(Medical Sciences);2004-04
4 WANG Jiexi1, GAO Xin1, BAI Yan2, REN Huiming1, TAN Yingxia1 & ZHANG Yangpei1 1. Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China; 2. Department of Hematology, The 307 Hospital, Beijing 10039, China Correspondence should be addressed to Zhang Yangpei;Rudimentary study on humanization of porcine red blood cells: Enzymatic removal of galactose-,3- galactose antigen from porcine red blood cell[J];科学通报(英文版);2003-04
5 LUAN Jianfeng1,GONG Feng2,ZHU Peiyuan1,YOU Ying2,LEI Qianhong1,YAN Jiangmei1,ZHANG Yangpei2(1.Department of Blood Transfusion,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002,China;2.Department of Blood Transfusion,Academy of Military Medical Sciences,Beijing 100850,China);Application of auto-cell washing system in conversion of blood type[J];Chinese Journal of Clinical Laboratory Science;2007-04
6 LIN Yi-gang,XIA Gang(Key Laboratory of Brain Functional Genomics,Ministry of Education,Shanghai Key Laboratory of BrainFunctional Genomics,East China Normal University,Shanghai 200062,China);Overexpression and characterization of a bacterial α-N-acetylgalactosaminidase[J];Journal of East China Normal University(Natural Science);2012-04
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10 WANG Jie Xi, ZHANG Yang Pei (Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850);Recent Advance on Blood Group Antigen Modification of Porcine Erythrocytes[J];Journal of Experimental Hematology;2002-03
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