Comparative analysis of the three-dimensional structure of Periplaneta fuliginosa densovirus
LI Li1, CHEN Donghua2,3, ZHOU Zhenghong3, ZHANG Jiamin1 & HU Yuanyang1 1. Institute of Virology, Wuhan University, Wuhan 430072, China; 2. Beijing Laboratory of Electron Microscopy, Chinese Academy of Sciences, Beijing 100080, China; 3. Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical School, Houston, Taxas 77030, USA
The three-dimensional structure of Periplaneta fuliginosa densovirus (pfDNV) is determined at 2.3 nm reso-lution using the techniques of cryo-electron microscopy and image reconstruction. The pfDNV contains five structural proteins and 60 protein subunits arranged on a T = 1 icosa-hedral shell with a relatively smooth surface. Its recon-struction reveals its distinct capsid structure from those ob-served in CPV and GmDNV. As in GmDNV, spike-like protrusions are not present in pfDNV at the threefold axes; while two small thorn-like protrusions are identified there. However, different from CPV and GmDNV, cylindrical channels along the fivefold axes are closed in pfDNV; while a small thorn-like protrusions, which have not been reported in other parvovirus, are observed there in pfDNV although their function is yet to be investigated. The pfDNV has dim-ple-like depressions at the icosahedral twofold axes; but has no canyon-like regions encircling the fivefold axes. The ico-sahedrally well-ordered nucleic acid has also been observed in pfDNV, suggesting that the protein and nucleic acid probably form closed interaction.
【CateGory Index】： Q933