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《科学通报(英文版)》 2003-05
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Effects of the disaggregation of high-polymerized particles in guard cell vacuoles on osmoregulation of stomatal aperture (stomata opening)

YE Qing, ZHU Guoli & LOU Chenghou Biology College, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China Correspondence should be addressed to Zhu Guoli (e-mail: glzhu@mail. cau.edu.cn)  
Observation under an electron microscope reveals that in closed and open stomata of V. faba, the aver-age volume of particles in guard cell vacuoles (GCV) reduces about 3 orders in magnitude, while the distribution density of the particles increases about 2 orders of magnitude. By using the method of the ratio of fluorescent emissions with laser scanning confocal microscopy, the monitoring to sto-mata opening shows that during 10 to 30 s before the first distinguishable aperture of stomata, there is a change of pH in GCV about -0.5 units. A quick stomatal opening immedi-ately follows the changes of pH in GCV to reach a steady aperture about 12 mm in 100200 s. This work proposes a model for the osmoregulation in GCV for stomatal opening. The proposed osmoregulation is related to the disaggregation of some polymerized particles inside GCV, which is probably induced by a -DpH in the vacuole. This model describes a process of osmoregulation that avoids the massive energy consuming transportation across cell membranes, which is a foundation of the current chemiosmotic hypothesis. This model is a supplement to the multiple controlling hypothesis for the stomatal movement, which widens research principle ideas for other quick movements in plants.
【Fund】: the National Natural Science Foundation of China (Grant Nos. 39870075 and 30170451) and The Major State Basic Research Development Program of China (Grant No. G19990117)
【CateGory Index】: Q945
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