PCR-RFLP analysis of mitochondrial DNA ND5/6 region among 3 subspecies of common carp (Cyprinus carpio L.)and its application to genetic discrimination of subspecies

ZHOU Jianfeng, WANG Zhongwei, YE Yuzhen & WU Qingjiang State Key Laboratory of Freshwater Ecology and Biotechnology, Insti-tute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China Correspondence should be addressed to Wu Qingjiang (e-mail: qjwu@ihb.ac.cn)  

Mitochondrial DNA ND5/6 region was studied by PCR-RFLP analysis among ten representative strains belonging to three subspecies (Cyprinus carpio carpio, Cypri-nus carpio haematopterus and Cyprinus carpio rubrofuscus) of common carp (Cyprinus carpio L.). A total of 2.4 kb frag-ment was amplified and subjected to restriction endonucle-ase analysis with nine restriction endonucleases subsequently.The results indicated that each subspecies owned one hyplo-type and four restriction enzymes (DdeⅠ, HaeⅢ, TaqⅠand MboⅠ) produced diagnostic restriction sites which could be used for discriminating the three subspecies and as molecu-lar genetic markers for assistant selective breeding of com-mon carp.

【Fund】： the Na-tional Natural Science Foundation of China (Grant No. 39830300) and the Key Laboratory of Freshwater Ecology and Biotechnology;; Institute of Hydrobiology;;the Chinese Academy of Sciences
【CateGory Index】： Q953

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