Effects of different nuclear recipients on developmental potential of mouse somatic nuclear transfer embryos
LEI Lei1,2, LIU Zhonghua1,3, ZHU Ziyu1,4, KOU Zhaohui1, WU Yuqi1, XU Ying1, WEN Duancheng1, BI Chunming1, XIA Guoliang1,2 & CHEN Dayuan1 1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China; 2. College of Biology, Agricultural University of China, Beijing 100094, China; 3. College of Animal Science, Northeast Agricultural University, Harbin 150030, China; 4. College of Life Science, Suzhou University, Suzhou 215006, China Correspondence should be addressed to Chen Dayuan (e-mail: Chendy@ panda.ioz.ac.cn)
In order to investigate the effects of different kinds of nuclear recipients from Kunming (KM) mouse on developmental potential of somatic nuclear transfer em- bryos, the enucleated MⅡ oocytes, enucleated zygotes and 2-cell blastomere were used to produce cloned mouse em-bryos. Using fibroblast deriving from C57/BL6 ear tissue as nuclear donor, we produced cloned embryos by transferring the fibroblast nuclei into enucleated KM mouse oocytes (sin-gle nuclear transfer, SNT), transferring pronuclei from the SNT embryos into enucleated KM zygotes (nuclear into zy-gote, NZ), and 2-cell blastomere nuclei from SNT embryos into enucleated KM mouse oocytes (nuclear into oocytes, NO); tetraploid embryos (tetraploid embryos, TE) were ob-tained by fusing two blastomeres, one is from the SNT cloned embryos, and the other from normal 2-cell KM mouse em-bryos. In group SNT, the cloned embryos could not develop beyond 8-cell stage and the rate of 8-cell stage is only 0.3%; in group NO, the reconstructed embryos could develop to morula stage, the rate of 8-cell stage was significantly greater than that of SNT group (P 0.05); in group NZ, the devel-opment rate was further improved, and the reconstructed embryos could develop into blastocyst stage, the rate of blastocyst was 1.9%; in group TE, as high as 62.3% of the reconstructed embryos could develop into blastocyst. Results suggested that different nuclear recipients could significantly affect the developmental potential of cloned mouse embryos; KM MⅡ oocyte cytoplasm was not so effective as zygotes to reprogram the mouse somatic cell nuclei; serial nuclear transfer could improve the developmental potential of cloned mouse embryos.
【Fund】： the Climbing Project of the Ministry of Science and Technology of China (Grant No. 97021109-2) and the Major Project of Knowledge Innovation of the Chinese Academy of Sciences (Grant No. KSCX1-05-01)
【CateGory Index】： Q953
【CateGory Index】： Q953