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《科学通报(英文版)》 2003-05
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Novel rAAV production system with low contamina-tion of helper virus

CHEN Li, CHEN Haoming, LI Ge, LU Daru & XUE Jinglun State Key Laboratory of Genetic Engineering, Institute of Genetics,School of Life Sciences, Fudan University, Shanghai 200433, China Correspondence should be addressed to Xue Jinglun (e-mail: jlxue@ fudan.ac.cn)  
The Cre-loxP system was introduced in theproduction of recombinant AAV (rAAV). Defective adenovi-rus AdLC was used as the helper virus. While doing themass production of recombinant AAV carrying EGFP orhuman clotting factor Ⅸ (hFⅨ) gene, the generation ofhelper virus was significantly limited, it increased the sim-plicity of rAAV purification. The results from in vivo study demonstrated the superiority of this method. This systemprovides a novel approach for the application of rAAV sys-tem in gene therapy.
【Fund】: the National 63High-tech Development Program (Grant No. 2001AA217181);;the Na-tional Natural Science Foundation of China (Grant No. 39880019);;Foundation of Doctor Degree Thesis from the Ministry of Education of China (Grant No. 199925) and Doctor Deg
【CateGory Index】: R373
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 Lai Lihui, Chen Li, Zhou Hong 1,Wang Jianmin 1,Hao Guangrong 2,Lu Daru,Sun Wei 2, Zhou Caihong,Qiu Xinfang, Xue Jinglun (Institute of Genetics, Fudan University; Changhai Hospital, Second Military Medical University 1; Animal Center, Second Milit;Studies on the Phenotype and Genetic Stability of Coagulation Factor Ⅸ-deficient Mouse Model for Human Hemophilia B[J];JOURNAL OF FUDAN UNIVERSITY;1999-04
2 LAI Lihui , CHEN Li , WANG Jianmin ZHOU Hong , LU Daru , WANG Qi GAO Xiaobo , QIU Xinfang and XUE Jinglun (1. Institute of Genetics, Fudan University, Shanghai 200433, China; 2. Hematology Department, Changhai Hospital, Second Military Medical University, Shanghai 200433, China);Skeletal muscle-specific expression of human blood coagulation factor IX rescues factor IX deficiency mouse by AAV-mediated gene transfer[J];中国科学(C辑:生命科学)(英文版);1999-06
3 LU Huazhong , CHEN Li WANG HongweiWU Zhijian WU Xiaobing WANG XuefengWANG Hongli LU Daru QIU Xinfang& XUE JinglunThe State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China;The State Key Laboratory of Molecular Virology and Gene Engineering, Chinese Academy of Preventive Medical Sciences, Beijing 100050, China;Shanghai Institute of Hematology, Ruijin Hospital of Shanghai Second Medical University, Shanghai 200025, ChinaPresent address: Laboratory for Blood Engineering, Shanghai Institute of Blood Transfusion, Shanghai Blood Center, Shanghai 200051.;Gene therapy for hemophilia B mediated by recombinant adeno-associated viral vector with hFIXR338A, a high catalytic activity mutation of human coagulation factor IX[J];中国科学(C辑:生命科学)(英文版);2001-06
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 HAO Guang Rong 1* , SUN Wei 1, HU Wei Jiang 1, CHEN Li 2, CUI Shu Fang 1, TANG Qiu 1, CAO Ping 1 (1. Laboratory Animal Center,Second Military Medical University,Shanghai 200433,China; 2. Institute of Genetics Research,Fudan University);Establishing inbred strain of coagulation factor Ⅸ-knockout mouse[J];Academic Journal of Second Military Medical University;2003-02
2 LU Huazhong , CHEN Li WANG HongweiWU Zhijian WU Xiaobing WANG XuefengWANG Hongli LU Daru QIU Xinfang& XUE JinglunThe State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China;The State Key Laboratory of Molecular Virology and Gene Engineering, Chinese Academy of Preventive Medical Sciences, Beijing 100050, China;Shanghai Institute of Hematology, Ruijin Hospital of Shanghai Second Medical University, Shanghai 200025, ChinaPresent address: Laboratory for Blood Engineering, Shanghai Institute of Blood Transfusion, Shanghai Blood Center, Shanghai 200051.;Gene therapy for hemophilia B mediated by recombinant adeno-associated viral vector with hFIXR338A, a high catalytic activity mutation of human coagulation factor IX[J];中国科学(C辑:生命科学)(英文版);2001-06
3 LU Huazhong1*, CHEN Li1, WANG Xuefeng2, LU Daru1, QIU Xinfang1 & XUE Jinglun (Jerry L. Hseuh)1 1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China; 2. Shanghai Institut;Preparation of a recombinant adeno-associated viral vector with a mutation of human factor-Ⅸ in large scale and its expression in vitro and in vivo[J];科学通报(英文版);2001-16
4 CHEN Li1, CHEN Haoming1, ZOU Beiyan1, WU Zhijian2, WU Xiaobing2, LU Daru1 & XUE Jinglun1 1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China;2. Institute of Virology, Chinese Academy of Preventive Medicine,Beijing 100052, China The first two authors contributed equally to this manuscript. Correspondence should be addressed to Xue Jinglun (e-mail: jlxue@fudan.ac.cn);Preparation of rAAV/hFⅨ by HSV/AAV hybrid helper virusand evaluation of its safety[J];科学通报(英文版);2003-13
5 Wei Jia-jun,Wang Zhuo-ran Department of Neurology,Renmin Hospital of Wuhan University,Wuhan 430060,Hubei Province,China;Package of recombinant adeno-associated virus encoding cell division cycle 2-siRNA[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2009-28
6 Xin-Yu Zhang, Sheng Tai, Zhi-Dong Wang, Shi-Bo Sun, De-Quan Wu, De-En Han, the First Department of General Surgery, the Second Clinical Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China Hong-Chi Jiang, Bei Sun, Department of General Surgery, the First Clinical Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China Ling-Wang Zhou, Endemic Disease Branch, Chinese Center for Disease Control, Harbin 150086, Heilongjiang Province, China;Construction of an adeno-associated viral vector serotype 2/1 containing human interleukin-10 and its expression in donor liver[J];World Chinese Journal of Digestology;2005-12
7 HAO Guangrong, SUN Wei, CHEN Li, CUI Shufang, TANG Qiu, HUANG Lizhi, CAO Ping (Laboratory Animal Center, Second Military Medical University,Shanghai 200433, China);Genetic Analysis and Blood Assay of Coagulation Factor -IX Knockout Mouse Strain[J];Acta Laboratorium Animalis Scientia Sinica;2002-04
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Lai Lihui, Chen Li, Zhou Hong 1,Wang Jianmin 1,Hao Guangrong 2,Lu Daru,Sun Wei 2, Zhou Caihong,Qiu Xinfang, Xue Jinglun (Institute of Genetics, Fudan University; Changhai Hospital, Second Military Medical University 1; Animal Center, Second Milit;Studies on the Phenotype and Genetic Stability of Coagulation Factor Ⅸ-deficient Mouse Model for Human Hemophilia B[J];JOURNAL OF FUDAN UNIVERSITY;1999-04
2 LU Da-Ru, ZHOU Jei-Min ZHENG Bing QIU Xin-Fang, XUE Jing-Lun(Institute of Genetics, Fudan Uniuersity, Shanghai 200433, PRC) WANG Jian-Min MENG Pei-Lin HAN Feng-Lai MING Bi-He (Department of Hematology, Changhai Hospital, Shanghai 200433, PRC )WANG Xiao-Peng, WANG Jian-Bo LIANG Jia-Jing and JIANG Zuo-Shu (Department of Biology, Second Military Medical University, Shanghai 200433, PRC);Stage Ⅰ Clinical Trial of Gene Therapy for Hemophilia B[J];中国科学B辑(英文版);1993-11
3 LAI Lihui , CHEN Li , WANG Jianmin ZHOU Hong , LU Daru , WANG Qi GAO Xiaobo , QIU Xinfang and XUE Jinglun (1. Institute of Genetics, Fudan University, Shanghai 200433, China; 2. Hematology Department, Changhai Hospital, Second Military Medical University, Shanghai 200433, China);Skeletal muscle-specific expression of human blood coagulation factor IX rescues factor IX deficiency mouse by AAV-mediated gene transfer[J];中国科学(C辑:生命科学)(英文版);1999-06
4 WU Zhijian, WU Xiaobing and HOU Yunde State Key Laboratory for Molecular Virology and Genetic Engineering , Institute of Virology , Chinese Academy of Preventive Medicine , Beijing 100052, China Corresponding author;Generation of a recombinant herpes simplex virus which can provide packaging function for recombinant adeno-associated[J];科学通报(英文版);1999-08
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