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《科学通报(英文版)》 2003-05
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Measurements of leucocyte membrane elasticity based on the optical tweezers

GUO Honglian1*, CAO Qinhong3*, REN Dongtao3, LIU Guoqing3, DUAN Jianfa2, LI Zhaolin1, ZHANG Daozhong1 & HAN Xuehai2 1. Optical Physics Laboratory, Institute of Physics and Center for Con-densed Matter Physics, Chinese Academy of Sciences, Beijing 100080, China; 2. State Key Laboratory of Biomacromolecles, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 3. State Key Loboratory of Plant Physiology and Biochemistry, College of Biology Sciences, China Agricultural University, Beijing 100094, China Correspondence should be addressed to Guo Honglian (e-mail: redlo-tus@aphy.iphy.ac.cn) or Cao Qinhong (e-mail: caoqinhong74@sina. com) * The first two authors have the same contributions to this paper.  
A 1-mm in diameter polystyrene bead coated with lectin is trapped by optical tweezers which is formed by a focused laser beam (740 nm). A leucocyte adhered to the bottom of sample cell is chosen to close to the trapped bead. When their stable combination is confirmed, the leucocyte is displaced by moving the sample stage. A tether is then formed between the trapped bead and the membrane. The force acting on the tether is measured by a detector equipped on the optical tweezers system. The deformation of the membrane is diverse in different contact conditions. When the contact area is small, the tether is very thin and the force on it is 7.4 pN (10-12 N), while the tether is much thicker un-der a larger contact area and its corresponding force is about 14 pN. It is presumed that the latter is related to the defor-mation of cytoskeleton.
【Fund】: the National Natural Science Foundation of China (Grant Nos. 1989380 and 10174097)
【CateGory Index】: Q73
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