A new method for studying caffeine's antioxygenic property: Peroxidase-Oxidase biochemical reaction
WANG Jun, CAI Ruxiu, LIN Zhixin & LIU Zhihong Department of Chemistry, Wuhan University, Wuhan 430072, China Correspondence should be addressed to Cai Ruxiu (e-mail: dlxfxc@ whu.edu.cn)
The effect of Caffeine on Peroxidase-Oxidase (PO) reaction was studied systematically in this paper. We proved that the valley of PO oscillation is the best phase an-gle which was used to research antioxygenic property by the Analyte Pulse Perturbation Technique (APP), based on in-vestigating the mechanism. Area integral calculus was pro-posed to use in quantitative analysis for the first time. There is a good linear relationship (R = 0.9950) between the ratio of amplitude changes of PO oscillation and the concentration of caffeine in the range 4.61×10-7 mol/L—1.84×10-5 mol/L. A new method for analysis by PO oscillation was set up. We also investigated two-dimensional projections and Fourier spectrum of nonlinear complicate system——PO reaction which was perturbed by caffeine, in order to provide a theo-retical basis for studying effects of kinds of antioxidants on life system.
【CateGory Index】： Q55