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《科学通报(英文版)》 2003-16
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Transgene for growth hormone in common carp (Cyprinus carpio L.) promotes thymus development

GUO Qionglin, WANG Yaping, JIA Weizhang & ZHU Zuoyan State Key Laboratory of Freshwater Ecology and Biotechnology, Insti-tute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China Correspondence should be addressed to Zhu Zuoyan (e-mail: zyzhu@ ihb.ac.cn)  
The transgenic carp were produced by micro-injection of CAgcGHc into the fertilized eggs. Observation of the thymus development between the transgenics and non- transgenic controls was carried out. The thymus of one-year- old transgenics F1 showed a great increase in both size and weight. The unilateral thymus of the transgenics weighed from 190 to 295 mg with average 218.6 mg, whereas the uni-lateral thymus of the controls weighed 20—81 mg with av-erage 42.5 mg; i.e. the thymus weight in the transgenics was 5.14 fold over that in the controls. The index of thymus/body weight in the transgenics was 2.97 fold over the controls. Light microscopy observation indicated that the thymus of the transgenics well developed with the thickened outer re-gion and compactly arranged thymocytes, while the thymus in the controls were degenerating with the thinned outer region, scattered thymocytes and groups of fatty cells. Fur-ther analysis with the electron microscopy revealed that pro-liferous cells in the transgenics were mainly small lympho-cytes and no pathological changes were found. The results confirmed that the 揂ll-fish?GH-transgene promotes thy-mus development and thymocyte proliferation, and retards thymus degeneration. The study has laid a foundation for further analysis of the immunobiological function in GH- transgenic carp.
【CateGory Index】: Q953
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