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《科学通报(英文版)》 2003-17
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Reconstruction of human embryos derived from somatic cells

LU Changfu, LIN Ge, XIE Changqing, GONG Fei, ZHOU Hong, TAN Yueqiu & LU Guangxiu Institute of Reproduction and Stem Cell Engineering of South University, Changsha, Hunan 410078, China  
Reconstruction of human nuclear transfer em-bryos is a necessary step of therapeutic cloning. In this study we injected somatic cell nuclei into MⅡ oocytes and acti-vated reconstructed oocytes with calcium ionophore A23187 (CaA) and 6-dimethylaminopurine (6-DMAP). After oocyte activation and 2PN formation, we removed the female PN. By using this method, we avoided the application of DNA fluorescent stain and ultraviolet light for oocyte enucleation, and over elimination of ooplasm was also mitigated. Some reconstructed embryos developed into the blastocyst stage in vitro.
【CateGory Index】: R329
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【Co-references】
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