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《Journal of Nanchang University(Medical Science)》 2012-08
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Detection of Immunoglobulin κ,λ Light Chain Restriction in Mantle Cell Lymphoma Using Immunohistochemistry and In Situ Hybridization

WAN Hong-ping,TU Lu-xia,SHENG Yi-yun,XIONG Zhen-fang,TAO Xue-qin, MEI Jin-hong (Department of Pathology,the First Affiliated Hospital of Nanchang University, Nanchang 330006,China)  
Objective To evaluate the application value of immunohistochemistry combined with in situ hybridization for detecting immunoglobulin κ,λ light chain restriction in mantle cell lymphoma(MCL).Methods The expression of CyclinD1 was detected by immunohistochemistry using Envision two step method in 15 cases of nodular mantle cell lymphoma(group 1),15 cases of diffuse mantle cell lymphoma group(group 2) and 10 cases of reactive lymph node hyperplasia(control group).The κ to λ light chain ratio was measured by in situ hybridization to determine the light chain restriction of tumor cells.Results CyclinD1 was positively expressed in nodular mantle cell lymphoma and diffuse mantle cell lymphoma(15/15,100.0%),but was not expressed in the surrounding residues or lymphocytes with reactive hyperplasia.In group 1,κ light chain restriction was found in 7 cases(7/15,46.7%),λ light chain restriction in 3 cases(3/15,20.0%),and no light chain restriction in 5 cases(5/15,33.3%).In group 2,κ light chain restriction was found in 2(2/15,13.3%),λ light chain restriction in 6 cases(6/15,40.0%),and no light chain restriction in 7 cases(7/15,46.7%).No cases showed light chain restriction in control group.Conclusion The light chain restriction and types can be detected by cyclin D1 immunohistochemistry and in situ hybridization in different types of MCL.Therefore,the combined detection is important for early diagnosis of MCL.
【Fund】: 江西省教育厅科技研究项目(赣教技字[2009117])
【CateGory Index】: R733.1
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHANG You *, Faith C.S. Ho, SHEN Danhua,XU Weisheng. CHAN Alexander C.L.,SRIVASTAVA Gopesh. Department of Pathology, Hong Kong University (*Department of Haematology, the Second Hospital of Kunming Medical College, Kunming 650101), China;Detection of Immunoglobulin light chain restriction of mucosa-associated lymphoid tissue type gastric lymphoma using mRNA in situ hybridization[J];Chinese Journal of Pathology;2001-01
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 Zhang You, Hu Dengming, Zhou Zeping. Department of Hematology/Lymphoma Unit of the 2nd Affiliated Hospital of Kunming Medical College (Kunming, 650101);DETECTION AND CLINICAL VALUE OF IMMUNOGLOBULIN KAPPA LIGHT CHAIN GENE REARRANGEMENT IN THE B CELL LYMPHOPROLIFERATIVE DISEASES[J];Journal of Leukemia & Lymphoma;2004-05
2 Li Chao,Zheng Xiongwei,Zhao Wenjin,Lu Lili.Department of Pathology,Fujian Provincial Tumor Hospital,Fuzhou 350014,China;To detect the EBV expression in lymphoma and its significance by the tissuechip technology[J];Fujian Medical Journal;2008-06
3 LI Weiqiang,YANG Zhou,and ZOU Yiyou.Department of Internal Medicine,Hunan Provinical Corps Hospital,Chinese People's Armed Police Forces,Changsha 410006,China;Expression of cyclin D_1,bcl-2 and CD95 proteins in gastric mucosa associated lymphoid tissue lymphoma[J];Medical Journal of the Chinese People's Armed Police Forces;2005-02
4 YANG Zhiwu,YANG Ling,ZHANG You.The Second Affiliated Hospital of Kunming Medical College,Kunming(650501). Objective;The study on immunoglobulin light chain detection in primary gastric lymphomas.[J];Medicine and Pharmacy of Yunnan;2002-06
5 LI Zhao-tao, CUI Xi-yu, HUANG Yan-xia(Department of Gastroenterlogy, the First people′s Hospital, Foshan, Guangdong 528000, P.R.China);Endoscopic features and clinical analysis of primary malignant lymphoma of stomach[J];China Journal of Endoscopy;2005-05
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