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《Journal of Liaoning Medical University》 2008-01
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Effects of TSA Acid on Proliferation in Thyroid Squamous Cell Carcinoma Cell Line SW579

WANG Xin XIAO Jianying ZHANG Xiumei LI Nan SONG Qiman Department of Biochemistry,Liaoning Medical University,Jinzhou 121001 China  
Objective To study the effects of trichostatin A(TSA)on apoptosis and proliferation of thyroid squamous cell carci- noma SW579.Methods Treated with different doses of TSA(final concentration of 25 nmol/L,50 nmol/L,100 nmoL/L,200 nmol/L,400nmoL/L),the cells were observed with an inverted microscope;The proliferation activities of cell line were determined by using methyl thiazolyl tetrazolium(MTT)assay.Cell morphologies and apoptotie were observed by fluorescence microscope stained with acridine orange.Flow cytometry was adopted to examine apoptotic rate.Results TSA inhibited significantly the growth of cell line and the effect was with a dose dependent manner.Typical apoptotic morphologic feature was discovered under fluorescence micro- scope.The apoptotic peak was detected with FCM and apoptotic rate increased with the concentration raised.Conclusions TSA can inhibit the proliferation and induce apoptosis in the thyroid cancer cells with a dose-dependent manner.
【Fund】: 辽宁省教育厅资助项目(编号:05L132)
【CateGory Index】: R736.1
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