Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Liaoning Medical University》 2009-01
Add to Favorite Get Latest Update

Influence of Age on Osteogenic Differentiation of Rat Adipose-derived Stem Cells

TAN Tao,QIN Shujian,WANG Chao,REN Guangda,PEI Dan(Department of Anatomy,Liaoning Medical University,Jinzhou 121001 China)  
Objective To investigate influence of ages on different osteogenic differentiation capability of rat adipose-derived stem cells(ADSCs).Methods ADSCs were isolated by using density gradient centrifuge method of different ages,and cultured in vitro.The adhesive cells were preserved to passage,the third generation of cell culture was for induction differentiation.All groups of cells were cultured with DMEM medium,which contains 0.1μmol/L Dexamethasone,10 mmol/L β-glycerophosphate and 50 μmol/L Ascorbic Acid.The morphology change,alkaline phosphatase activity and calcium ion assaying were observed.Results The results showed that in vitro ADSCs showed fusiform.The passage cells had high ADSCs proportion.Induced conditions morphology changed from fusiform into cubic shape and polygon,after the culture in the differentiation induction medium,alkaline phosphatase activity and calcium ion decreased with age.Conclusions Results indicate osteogenic differentiation capability of ADSCs decreases with aging.These findings have significant implications for the proposed clinical application of ADSCs.
【Fund】: 辽宁省科技计划项目(NO.2006408002-5)
【CateGory Index】: R329.28
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 QU Rong-mei*, YAO Hong-wei, DAI Jing-xing, CHEN Bai-hong, ZHANG Yan-ling, LIN Lai-xing-mei, YUAN Lin. *Center of Teaching and scientific research, School of biotechnology, South Medical University, Guangzhou 510515, China;The Comparison of differentiation potency of adipose-derived stem cells from sprague-dawley rats and wistar rats[J];The Journal of Practical Medicine;2011-07
Chinese Journal Full-text Database 10 Hits
1 YANG Ya-Jun,ZHU Qing-Sheng Institute of Orthopedics,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China;Differentiation of rat adipose-derived stem cells into chondrogenic phenotype with induction of cartilage-derived morphogenetic protein (CDMP1) growth factor in vitro[J];Journal of the Fourth Military Medical University;2008-04
2 ZHANG Xue-quan, YUAG Lin, YANG Lin-lin, JANG Xue-mei, YANG Chun, YU Lei, DAI Jing-xing Department of Human Anatomy, South Medical University, Guangzhou 510510, China;Biocompatibility between human adipose-derived mesenchymal stem cells and porcine bone scaffolds[J];Journal of Southern Medical University;2009-04
3 LI Dong-fei1,YANG Chun1,LI Zhen1,YUAN Ling-ling2,DAI Jing-xing1,YUAN Lin1 Department of Human Anatomy1,School of Basic Medical Sciences2,Southern Medical University,Guangzhou 510515,China;Immunophenotypic comparison of adipose-derived mesenchymal stem cells from the greater omentums and subcutaneous adipose tissues of rats[J];Journal of Southern Medical University;2010-10
4 ZHANG Yong reviewing,ZHAO Jian-ning checking(Department of Orthopedics,Jinling Hospital,Nanjing 210002,Jiangsu,China);Progress in researches of adipose-derived stem cells[J];Journal of Medical Postgraduates;2009-10
5 YUAN Lin1, WANG Jun1, WANG Chun-lei1, SHEN Bao-lin1, DAI Jing-xing1, HUANG Yong21. Teaching and Research Section of Anthropotomy, Southern Medical University, Guangzhou 510515,China2. Teaching and Research Section of Acupuncture and Moxibustion, School of Chinese Medicine,Southern Medical University, Guangzhou 510515, China;Fasciology: A New Theory on the Human Self-supervision and Control System[J];Science & Technology Review;2006-06
6 LI De-yuan,MU De-zhi(Department of Pediatrics,West China Second University Hospital,Sichuan University,Chengdu 610041,Sichuan,China);Biological characteristics and multipotential differentiation of adipose tissue-derived stem cells[J];Biomedical Engineering and Clinical Medicine;2008-06
7 Bai Yu1,Yuan Lin1,Huang Yong2,Wu Jinpeng1,Wang Jun1,Dai Jingxing1,Wang Chunlei2,Jiang Xuemei1,Li Dongfei1,Yang Chun1,Yu Meichun1,Yang Huiying1,Tao Hui1,Sha Ou3,Yew David Tai Wai3(1.Department of Anatomy,Southern Medical University,Guangzhou 510515,China;2.Department of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China;3.Department of Anatomy,Chinese University of Hong Kong,Hong Kong,China);Anatomical Discovery of Meridians and Collaterals Lead to the New Theory of Fasciaology[J];World Science and Technology(Modernization of Traditional Chinese Medicine and Materia Medica);2010-01
8 LI Dong-fei, YANG Chun, LI Zhen, DAI Jing-xing, YU Mei-chun, YANG Hui-ying, TAO Hui, YUAN Lin. Department of Human Anatomy, South Medical University, Guangzhou 510515, China;Effects of skin wound on adipose-derived mesenchymal stem cells harvested from subcutaneous adipose tissue in rats[J];The Journal of Practical Medicine;2010-16
9 Wang Hongxiang,Zhao Shi,Mao Hong et al Department of Hematology,Wuhan Central Hospital,Wuhan 430014;Effects of Adipose Stromal Cells on Allo-genetic Dendritic Cells and Its Possible Role[J];Acta Medicinae Universitatis Scientiae et Technologiae Huazhong;2008-01
10 Li Dong-fei,Yang Chun,Li Zhen,Dai Jing-xing,Yuan LinDepartment of Human Anatomy,South Medical University,Guangzhou 510515,Guangdong Province,China;Immunophenotype determination of rat adipose tissue-derived mesenchymal stem cells at passage 4[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2010-23
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved