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《Journal of Liaoning Medical University》 2009-01
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Application of AFM Astrocytoma,Anaplastic Astrocytoma in the Differential Diagnosis

ZHANG Jiayi,JI Xiaolong,WANG Mei'e(Liaoning Medical University,Jinzhou 121001 China)  
Objective To make use of atomic force microscope to detect the difference and change of II/ WHO,III/WHO in the surface structures of cell membrance,then to provide help and guide for the clinical differential diagnosis between Astrocytoma and Anaplastic astrocytoma.Methods Between Astrocytoma and Anaplastic astrocytoma,we choose 30 examples from each.10 cells each example was chosen randomly and scanned with AFM using tapping mode in the range of 1 μm,the surface of cell membranes being measured in terms of rough,peak value,as well as difference of surface area.We observed the Frame changes in the two groups to find out the morphologic features.Results We found out the surface of cell membrane in the two groups had significant differences on rough,peak value,and difference of surface area.The Astrocytoma cells were significantly lower than Anaplastic astrocytoma cells(P0.05).Conclusions In our experiment,we can observe the Astrocytoma and Anaplastic astrocytoma cells with AFM,then make a differential diagnosis with the data of the surface of cell membranes.
【CateGory Index】: R739.4
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