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《Journal of Liaoning Medical University》 2013-04
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The Protective Effects of Tranilast on Cardiomyopathy in Diabetic Rats

JIANG Yu,FU Lijuan(Department of Pharmacology,Liaoning Medical University,Jinzhou 121000 China)  
Objective To observe the effects of tranilast on myocardial fibrosis and expressions of transforming growth factor-β1 in diabetic rats and investigate the protective effect and mechanism of tranilast on cardiac muscle in diabetic rat.Methods Among the forty healthy SD rats,ten were randomly selected as the normal control group(NC),and the rest thirty rats were established as diabetesmellitus rat models by tail vein injection of 30 mg.kg-1 1streptozotocin once.The diabetesmellitus rat models were randomly divided into diabetic model group(DM),low dose of tranilast(tra1) and high dose of tranilast therapy group(tra2).Each therapy group contained 10 rats.Tranilast of 100,200 mg /(kg.d) was respectively given to the low,high doses of drug therapy groups by gavage for 12 weeks.Rats’blood glucose and heart quality index(HMI) were calculated.Morphological change was observed and myocardial collagen volume fraction(CVF) was measured by Masson staining and the protein expression level of TGF-β1 was determined by immunohistochemistry.Results Compared with the NC group,the blood glucose and HMI of DM were significantly increased(P 0.01),± dp / dtmax was significantly reduced(P 0.01),while compared with the DM,HMI was reduced and ± dp / dtmax was significantly up-regulated(P 0.05).The cardiac muscle fibers of DM were in derangement,collagen fibers were increased,and the level of CVF was significantly up-regulated(P 0.01).Compared with the DM,HMI and ± dp / dtmax of tranilast treatment groups were improved and CVF showed different degrees of decline(P 0.01).Compared with the NC,the protein expression level of TGF-β1 was significantly up-regulated(P 0.01).Compared with the DM,the protein expression level of TGF-β1 of tranilast treatment groups was reduced(P 0.01).High dose of tranilast group(tra2) had been improved obviously(P 0.01).Conclusions Tranilast can retard the myocardial fibrosis and improve the cardiac remodeling.The protective mechanism may be related to the decrease of the protein expression level of TGF-β1.
【Fund】: 辽宁省科技计划项目 编号:2011225015
【CateGory Index】: R587.1
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