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《Journal of Liaoning Medical University》 2016-01
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Study of the Differentiation of Muscle- derived Stem Cells to Insulin- producing Cells Induced by Collagen Scaffold

LIU Rui;LIU Chang;YIN Tiantian;The First Affiliated Hospital of Liaoning Medical University;  
Objective To study the possibility that the neonatal rat muscle- derived stem cells( MDSCs) trans- differentiate to insulin- producing cells when collagen scaffold is added in culture medium and its possible mechanism. Methods MDSCs were extracted and purified from rats' skeletal muscles and identified with Desmin immunocytochemical staining. The MDSCs were then divided into 2 groups: collagen scaffold group and control group. The differentiation was induced and the cell morphology was observed. The insulin IPCs can be identified by dithizone( DTZ) staining,and reverse transcription polymerase chain reaction( RT PCR) was used to evaluate gene expression. Results Highly purified MDSCs were harvested and purified MDSCs were induced in these 2 groups. Expressions of the precursor islet cells related genes Nestin,PDX- 1 was detected by RT PCR in pancreas extract- induced group on the6 thday,and the typical islet like cell clusters were formed and insulin( INS) expression was detected on the 12 thday after induction.Conclusions Chemical methods can induce differentiation of MDSCs to IPCs by simulating the three- dimensional microenvironment of islet cell development in Vitro.
【Fund】: 辽宁省教育厅一般项目;编号:L2012304
【CateGory Index】: R587.1
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