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《Journal of Jinzhou Medical University》 2017-01
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Quantitative Determination of Scutellarin in the Water Decoction of Scutellaria Barbata D. Don and Hedyotis Diffusa Willd

SHI Jinping;WANG Yubo;ZHANG Yaqiu;FANG Yu;Jinzhou Medical University;  
Objective To establish the quantitative determination method of scutellarin in the water decoction of Scutellaria barbata D. Don and Hedyotis diffusa Willd with the ratio of one to one. Methods HPLC was used in the quantitative determination of the water decoction of Scutellaria barbata D. Don and Hedyotis diffusa Willd. The chromatographic condition was Diamonsil( 4. 6 mm×250mm,5 μm) chromatographic column,with a mixture of methanol-0. 1% phosphori1 c acid solution phosphate( 30 ∶ 70) as mobile phase. Results The method was good in linaer. The average recovery rate was 99. 8% and RSD was 1. 7%. Conclusion The method is of high precision,and the degree of separation is nice. It is a good method to determinate the water decoction of Scutellaria barbata D. Don and Hedyotis diffusa Willd.
【Fund】: 辽宁省大学生创新创业训练计划项目 项目编号:201410160040
【CateGory Index】: R284.1
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