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《Research and Practice on Chinese Medicines》 2013-01
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Research on Application of Cryo-sectioning Technique in Making Permanent Sections of Pharmaceutical Botany Tissues

ZHANG Jian-kui,WANG Bing,Xu Liang,KANG Ting-guo*(Liaoning University of TCM,Dalian 116600,China)  
Objective To establish the methods of making permanent sections of pharmaceutical botany tissues by cryo-sectioning technique.Method The procedure consisted of several steps as follows: The materials were embedded after fixed by FAA fixation liquid,then frozen and sliced by cryostat microtome betweent-18℃and-20℃.Result Sections were dehydrated in ascending series of ethanol,dyed with safranine and fast green,cleared in xylene and sealed with canada balsam.Structure of the section was intact and clear.Conclusion Cryo-section technology was a simple and efficient method to make permanent sections of plant tissues,it has broad prospect of application.
【Fund】: 国家自然基金资助项目(81173499)
【CateGory Index】: R283.3
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