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《Research and Practice on Chinese Medicines》 2013-01
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Determination of Aflatoxin G2,G1,B2,B1 in 34 Batches of Chinese Herbs by HPLC Associated with Post Column Photochemical Derivatization

YANG Wen-wu1,2,XIONG Ling-yun2,WANG Rui-fang2,Liu-Yan2,SUN Qi-sheng2,LEI-Yu2, WANG-Qiang1*(1.China Pharmaceutical University,Nanjing 210009,China;2.Tianjin Tasly Modern TCM Resources co.,ltd.,Tianjin 300402,China)  
Objective To establish HPLC methods associated with post column Photochemical Derivatization to determine aflatoxin G2,G1,B2,B1 in Chinese herbal.Method Aflatoxins were extracted by 70% methanol and purified by an immunoaffinity column.Then the samples were analysed by HPLC fluorescence detector with post column Photochemical derivatization.Confirm the suspected components with LC-MS.Results The method with the great linear concentration range of 0.75-22.5 pg for aflatoxins G2,B2,and 5-75 pg for aflatoxins G1,B1 respectively,was stable and accurate.In the result of 34 batches of Chinese herbs,Aflatoxins were detected in 3 batches of Semen Ziziphi Spinosae among which Aflatoxin B1 of one batch exceed 5 μg·kg-1.Conclusion The method is simple and accurate,which is suitable for the determination of aflatoxins in Chinese herbal.
【CateGory Index】: R282.5
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