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《Research and Practice on Chinese Medicines》 2013-01
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Content Determination Of Paeoniflorin and Tanshinone ⅡA in Jingzhi Guanxin Tablet by HPLC

CHEN Ying1,WANG Wen-yuan1,YANG Hua2(1.Hunan Yong-Zhou Vocational Technical college,YongZhou 425000,China.2.Hunan Yong-Zhou Institute for Drug Control,Yong-Zhou 425006,Chian)  
Objective To establish a method for content determination of paeoniflorin and tanshinone ⅡA in Jingzhi Guanxin Tablet by HPLC.Methods C18 colum(4.6 mm×250 mm,5 μm) was used with the mobile phase consisted of acetonitrile-0.2% phosphoric acid gradient elution,with flow rate 1.0 mL/min and the detection wavelength 270 nm(detection for tanshinone ⅡA),the detection time 10 min,230nm(detection for paeoniflorin),and the column temperature 25℃.Results The linear range for Paeoniflorin and tanshinone ⅡA were 12.52-125.2 μg·mL-1(r=0.999 8) and 2.67-26.7μg·mL-1(r=0.999 9),the average recovery was 100.1%(RSD=2.4%,n=6) for Paeoniflorin and 100.2%(RSD=1.8%,n=6)for tanshinone ⅡA.Conclusion The method was feasible,accurate and reliable.It could be used for the quality control of Jingzhi Guanxin Tablet.
【CateGory Index】: R286.0
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