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《Research and Practice on Chinese Medicines》 2013-01
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Content Determination of AstragalosideⅣ in Hua Sheng Ping Composition by RP-HPLC

WANG Na1,XI Zhi-xia1,CHE Xiao-xia1,WU Wen-ying1,XU Xiao-na2,FENG Wei1(1.Department of Pharmacy,The Second Affiliated Hospital,School of Medicine,Xi'an Jiaotong University,Xian 710004,China;2.Department of Pharmacy,School of Medicine,Xi'an Jiaotong University,Xi'an 710049,China)  
Objective To establish the method for determination of astragalosideⅣ in Hua Sheng Ping composition.Method The contents of astragalosideⅣ in Hua Sheng Ping composition were determined by HPLC.Kromasil C18 column(4.6 mm×250 mm,5 μm) was used with acetonitrile-water-phosphoric acid(1:2:0.15) as mobile phase,and the detective wavelength was 203nm.The flow rate was 1.0 mL·min-1 and the column temperature was room temperature.Results AstragalosideⅣ had a good linear relation in the range of 0.5-10μg(r=0.999 6) and the average recovery was 97.82%,RSD was 1.79%.Conclusion The method has good precision and reproducibility,and the results were reliable and accurate.It could be used to determine the contents of astragalosideⅣ in Hua Sheng Ping composition.
【CateGory Index】: R286
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