Solid-phase microextraction of pheromone components of Dendrolimus houi(Lepidoptera:Lasiocampidae) and their characterization by gas chro-matography-mass spectrometry and GC-electroantennographic detection
KONG Xiang-Bo1,ZHAO Li-Lin2,ZHANG Zhen1,WANG Hong-Bin1,BAI Fang-Hua3,YU Guo-Min4(1. Key Laboratory of Forest Protection of State Forestry Administration,Research Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091,China; 2. Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China; 3. Jinsishan Forest Farm of Dongzhi County of Anhui Province,Dongzhi,Anhui 247200,China; 4. Forestry Bureau of Keshiketeng Banner of Inner Mongolia,Keshiketeng,Inner Mongolia 025350,China)
With a close-range live specimen sampling technique based on solid-phase microextraction (SPME),the pheromone components of Dendrolimus houi virgin female moths during the calling period were collected in order to increase the quantities of pheromone components. The sample-loaded SPME fiber was introduced onto the gas chromatography (GC) coupled with mass spectrometry (MS) or electroantennographic detection (EAD),and the samples were analyzed under oven temperature program condition. The results indicated that large amounts of (5E,7Z)-5,7-dodecadien-1-ol (E5,Z7-12:OH) and (5E,7Z)-5,7-dodecadien-1-yl acetate (E5,Z7-12:OAc) were obtained by SPME CAR/DVB fiber,and trace amounts of (5E,7Z)-5,7-dodecadienal (E5,Z7-12:Ald) were also observed in the effluvia. The three compounds sampled by SPME fiber could evoke electroantennographic responses from male moth antennae. Compared with the pheromone gland extraction method with solvent,SPME sampling technique coupled with GC-MS and GC-EAD analysis has some advantages,such as representative sample collecting,relative large quantities of sample obtained,and solvent-free sample preparation technique,etc. Finally,the importance and practical aspects concerning the SPME are also discussed.