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《Science & Technology Review》 2015-01
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Laboratory diagnosis of Ebola virus disease:A review

QIAO Jinjuan;LUO Jun;WEI Hongping;Center for Emerging Infectious Diseases,Wuhan Institute of Virology,Chinese Academy of Sciences;University of Chinese Academy of Sciences;  
Ebola virus disease(EVD) caused by Ebola viruses(EBOV) is an acute hemorrhagic infectious disease. Because of its high infectivity and high mortality rate(up to 90%),EBOV is listed as one of the most dangerous viruses to human by World Health Organization. Although US FDA gave it emergency clearance to use an experimental drug TKM-Ebola,there is no Ebola vaccine available yet. Rapid,accurate,simple and reliable laboratory diagnostic tests are required in suspected cases for timely treatment,epidemiological investigation and controlling the spread of the EVD. This review summarizes the principles,applications and progress of the laboratory diagnosis of EVD,covering virus isolation by cell culture,electron microscopy,reverse transcriptase polymerase chain reaction(RT-PCR) assay,antigen detection,antibody-capture enzyme-linked immunosorbent assay and serum neutralization test.
【Fund】: 中国科学院重点部署项目(KJZD-EW-L02);; 武汉新发传染病与生物安全重点实验室项目(WHEID-2014004)
【CateGory Index】: R512.8;R446.5
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 WANG Shu-jie1,WANG Xi-jun1,HU Sen1,QIN Li-ting2,LIN Xiang-mei2,BU Zhi-gao1 1.National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China;2.Chinese Academy of Inspection and Quarantine,Beijing 100025,China;Development of an antigen capture ELISA against VP40 protein of Ebola virus using monoclonal antibodies[J];Chinese Journal of Preventive Veterinary Medicine;2008-04
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【Co-citations】
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1 PEI Shi-chun, LI Yu-hua, ZHANG Yuan-yuan, et a(lNortheast China University of Forestry, Harbin 150040, China);Preparation and Application of Heavy Chain IgG2b Isotype McAb against Aflatoxin B1[J];Chinese Journal of Biologicals;2009-06
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6 GAO Wei;ZHAO Ping;REN Hao;QI Zhong-Tian;Department of Microbiology,The Second Military Medical University,Shanghai Key Laboratory of Medical Biodefense;;Laboratory detection for Ebola virus infection[J];Journal of Microbes and Infections;2014-04
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