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《Chinese Science Bulletin》 2010-01
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Microplate chemiluminescence immunoassay for determination of triiodothyronine in human serum

WANG ALian1,2,XIAO Qin2,YING XiTang3,CHEN GuoNan1,LIN JinMing2 1College of Chemistry and Chemical Engineering,Fuzhou University,Fuzhou 350002;2Department of Chemistry,Tsinghua University,Beijing 100084;3Beijing Chemclin Biotech Co.,Ltd.,Beijing 100094  
A microplate chemiluminescence immunoassay(CLIA) was developed for the clinical determination of Triiodothyronine(T3) in human serum,and the luminal-hydrogen peroxide was used as chemiluminescence system catalyzed by horseradish peroxidase(HRP).Several physicochemical parameters such as the dilution ratios of coated antibody and HRP-T3 conjugate was chose,the incubation time and detection time were optimized.Methodology evaluation was done:the detection limit was 0.07 ng/mL;intra-assay and inter-assay coefficient of variation(CV) was less than 10%;the recovery was 80%-90%;there was not have obviously cross-reactivity of T4 and rT3 with the similar structure.Compared with a commercial CLIA kit,there was a good correlation between the two methods,the correlation coefficient was 0.9547.
【CateGory Index】: R446.6
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