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《Chinese Science Bulletin》 2010-Z2
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Plasmon resonance determination of ATP on the basis of its coordination with the surface of positive-charged gold nanoparticles

LI ChunMei1, LI YuanFang1, WANG Jian1 & HUANG ChengZhi1,2 1Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China; 2College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China  
It was found adenosine triphosphate (ATP) could induce the aggregation of cetyltrimethylammonium bromide (CTAB) capped gold nanoparticles (AuNPs) in Tris-HCl buffer pH 8.0, which caused the changes of surface plasma resonance absorption and resonance light scattering. Scanning electron microscopy (SEM), dynamic light scattering (DLS) and ζ-potential have been used to characterize the aggregation of AuNPs, and how ATP molecule combine with AuNPs has been discussed. With the changes of surface plasmon resonance absorption of AuNPs, a new method for the determina-tion of ATP has been developed in linear range of 4.0–80 μmol/L, with the detection limit of 0.82 μmol/L. It was found that ADP, AMP, UTP, CTP, GTP had no interfering effects in the determination. The method could be applied for the determination of ATP in urine samples with the recovery between 94.4% and 123%, and R.S.D. less than 2.5%. Com-paring with other methods, the proposed method reported here is simple, rapid, and highly selective.
【Fund】: 国家自然科学基金重大研究计划资助项目(90813019)
【CateGory Index】: O641.4
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