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《Journal of Clinical Otorhinolaryngology》 2006-13
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Excitotoxic effects of glutamate on the in vitro spiral ganglion cells

YAN Kaisheng XUE Qiuhong LUO Linghui GONG Shusheng (Department of Otolaryngology, Union Hospital, Huazhong University of Science and Techno- logy , Wuhan,430022, China)  
Objective:To explore the excitotoxic effects of glutamate on the in vitro primarily cultured spiral ganglion cell (SGC) of rat.Method:Spiral ganglion cells (SGCs) were cultured in vitro for 4 days, and exposed to 1mmol/L glutamate for 24 hours. Damaged cells double-labeling with Hoechest33258 and PI were observed by fluorescence microscope. Ffluo-3 and CLSM for measurement of intracellular calcium levels were also utilized.Result:Most cells were damaged and the intracellular calcium increased and after exposure to 1 mmol/L glutamate(P 0.05 ).Conclusion:Glutamate excitotoxicity was associated with free intracellular calcium ion concentration elevation in SGC.
【Fund】: 国家自然科学基金资助项目(No:30471879)
【CateGory Index】: R764.35
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