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《Journal of Clinical Otorhinolaryngology Head and Neck Surgery》 2008-21
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Apoptosis of laryngeal cacinoma cells induced by antisense oligodeoxynucleotides complementary Stat3 in vitro

LV Haili ZHANG Qiuhang YAN Bo(Department of Otolaryngology-Head and Neck Surgery, Xuanwu Hospital, Capital University of Medical Science,Beijing ,100053,China)  
Objective:To study the effects of oligodeoxynucleotides complementary Stat3 on apoptosis in laryngeal carcinoma Hep-2 cell. Method:Oligodeoxynucleotides complementary Stat3 was designed, which was transferred into laryngeal carcinoma Hep-2 cell by lipofection. Expression of Stat3 and p-Stat3 were detected by Western blot and PCR. MTT was used to observe the growth-inhibiting ratio. DNA ladder, AO/EB and FCM were used to observe the apoptosis of laryngeal carcinoma cell Hep-2 in vitro. Result:Western blot and PCR results demonstrated that oligodeoxynucleotides complementary Stat3 could significantly inhibit the expression of Stat3 and p-Stat3 in Hep-2 cell. MTT results showed that it could significantly suppress the growth of Hep-2 cell. The DNA ladder, AO/EB and FCM results showed it could inhibit the expression of Stat3 and induce the apoptosis of Hep-2 cell in a concentration-dependment manner. Conclusion:Oligodeoxynucleotides complementary Stat3 could induce the apoptosis and suppress cell proliferation in laryngeal carcinoma Hep-2 cell.
【CateGory Index】: R739.65
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【References】
Chinese Journal Full-text Database 3 Hits
1 L Haili ZHANG Qiuhang YAN Bo(Department of Otolaryngology-Head and Neck Surgery,Xuanwu Hospital,Capital University of Medical Science,Beijing,100053,China);Stat3 antisense oligodeoxynucleotide induces apoptosis in laryngeal carcinoma cells by regulating apoptosis-related factors[J];Journal of Clinical Otorhinolaryngology Head and Neck Surgery;2010-04
2 LIU Ting-yan 1,PENG Hua-guang 1,WU Zheng-hu 1,ZHANG Mei-xia 2 1Department of Otolaryngology-Head and Neck Surgery,Affiliated Hospital of Hangzhou Normal University,Hangzhou 310015,China;2Department of Clinical Pharmacology,China Medical University,Shenyang 110001,China;Expression of p-STAT3 and Skp2 and their correlation in laryngeal carcinoma[J];Journal of Modern Oncology;2010-03
3 LUO Hui-xiu,SUN Jie,XUE Wei-guo,et al. (Department of Otorhinolaryngology,Affiliated Qingdao Municipal Hospital of Qingdao University,Qingdao 266011,China);Expression and significance of STAT3 in nasal-sinuses squamous carcinoma[J];Chinese Journal of Otorhinolaryngology-Skull Base Surgery;2009-06
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 Li-fang GAO~1 De-qi XU~2 Lian-ji WEN~3 Xing-yi ZHANG~4 Yue-ting SHAO~1 Xue-jian ZHAO~(1,5)1 Department of Pathophysiology,Basic School of Medicine,Jilin University,Changchun 130021,China;2 Laboratory of Enteric and Sexually Transmitted Diseases,Center for Biologics Evaluation and Researeh,Food and Drug Administration,Bethesda MD,20892,USA;3 Departments of ENT4 Thoracic and Cardiovascular Surgery,the Second Hospital of Jilin University,Changchun 130021,China;Inhibition of STAT3 expression by siRNA suppresses growth and induces apoptosis in laryngeal cancer cells[J];中国药理学报(英文版);2005-03
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