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《Journal of Clinical Otorhinolaryngology Head and Neck Surgery》 2008-21
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Establishment of a cisplatin-induced human nasopharyngeal carcinoma drug-resistant cell line and its biological characteristics

LIN Wen1 LI Derui2 LIN Yingcheng1 DU Caiwen1 CHEN Jiongyu3 HONG Chaoqun3(1Department of Medical Oncology, Affiliated Cancer Hospital, Shantou University Medical College, Shantou, 515031, China;2Department of Radiation Oncology, Affiliated Cancer Hospital, Shantou University Medical College;3Laboratory of Cancer Research, Affiliated Cancer Hospital, Shantou University Medical College)  
Objective:To establish a CDDP-resistant cell line from human nasopharyngeal carcinoma and evaluate its biological characteristics. Method:By continuously exposing and gradually increasing dose of cisplatin (CDDP), a resistant nasopharyngeal carcinoma cell line (HNE1/CDDP) was established. Drug sensitivity of this cell line was detected by MTT assay; the alterations of its biological characteristics were determined using light microscopy, cell counting and flow cytometry (FCM); its ability of adhesion, migration and invasion were also evaluated. Result:HNE1/CDDP cell line was developed after 10 months with stable resistance to cisplatin with the resistance index was 5.83. HNE1/CDDP cell exhibited cross-resistance to many other chemotherapeutic agents (carboplatin, oxaliplatin and etoposide, etc). The morphology of HNE1/CDDP changed; doubling time prolonged; and the cell number of S-phase and G2/M-phase decreased while of G0/G1 phase increased compared with parental cells. The ability of adhesion, migration and invasion had no difference between the parental and the resistant cells. Conclusion: HNE1/CDDP cell line shows the typical and stable resistant phenotype and can be used as a research model.
【Fund】: 广东省医学科研基金(No:B2005099)
【CateGory Index】: R739.63
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