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《Journal of Clinical Otorhinolaryngology Head and Neck Surgery》 2008-21
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Establishment of a cisplatin-induced human nasopharyngeal carcinoma drug-resistant cell line and its biological characteristics

LIN Wen1 LI Derui2 LIN Yingcheng1 DU Caiwen1 CHEN Jiongyu3 HONG Chaoqun3(1Department of Medical Oncology, Affiliated Cancer Hospital, Shantou University Medical College, Shantou, 515031, China;2Department of Radiation Oncology, Affiliated Cancer Hospital, Shantou University Medical College;3Laboratory of Cancer Research, Affiliated Cancer Hospital, Shantou University Medical College)  
Objective:To establish a CDDP-resistant cell line from human nasopharyngeal carcinoma and evaluate its biological characteristics. Method:By continuously exposing and gradually increasing dose of cisplatin (CDDP), a resistant nasopharyngeal carcinoma cell line (HNE1/CDDP) was established. Drug sensitivity of this cell line was detected by MTT assay; the alterations of its biological characteristics were determined using light microscopy, cell counting and flow cytometry (FCM); its ability of adhesion, migration and invasion were also evaluated. Result:HNE1/CDDP cell line was developed after 10 months with stable resistance to cisplatin with the resistance index was 5.83. HNE1/CDDP cell exhibited cross-resistance to many other chemotherapeutic agents (carboplatin, oxaliplatin and etoposide, etc). The morphology of HNE1/CDDP changed; doubling time prolonged; and the cell number of S-phase and G2/M-phase decreased while of G0/G1 phase increased compared with parental cells. The ability of adhesion, migration and invasion had no difference between the parental and the resistant cells. Conclusion: HNE1/CDDP cell line shows the typical and stable resistant phenotype and can be used as a research model.
【Fund】: 广东省医学科研基金(No:B2005099)
【CateGory Index】: R739.63
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 YANG Jun-xia1*,TANG Wei-xue2 1.Department of Pharmacology,2.Department of Pathophysiology,Chongqing Medical University,Chongqing 400016,P.R.China;Establishment of a Cisplatin-Induced Human Hepatocellular Carcinoma Drug-Resistant Cell Line and Its Bio logical Characteristics[J];Chinese Journal of Cancer;2002-08
2 ;Cell adhesion and tumor multicellular drug resistance[J];Acta Academiae Medicine Militaris Tertiae;2002-11
3 LI Yan, WU Jun-Zheng, SITU Zhen-Qiang, LIU-Bin, WANG Jiang-Hua, LI-Feng, LI-Jie Department of Oral Biology, Stomatological College, Fourth Military Medical University, Xi'an 710033;Establishment and characterization of a multidrug resistant cell clone derived from human salivary gland mucoepidermoid carcinoma cell line MEC-1[J];Journal of The Fourth Military Medical University;2002-18
4 CHENG Guojun, LI Yali, TIAN Fang, et al. Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Chinese Academy of Military Medical Sciences, Beijing 100853, China;Comparison of stepwise and pulse induced cisplatin-resistant ovarian cancer cell sublines[J];Chinese Journal of Oncology;2001-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Bao-xia 1、2 , YU Zhi-hong 3, FAN Kai 1, YAN Ping 4,ZHANG Chao 1, L Shen 1、3 (1.Department of Pathology, Dalian Medical University, Dalian 116027, China; 2.College of Laboratory Medicine, Dalian Medical University, Dalian 116027, China; 3.Laboratory of Molecular Biology, the Second Hospital of Dalian Medical University, Dalian 116027, China; 4.Center of analyse and test, Dalian Medical University, Dalian 116027, China);Loss expression of hMSH2 with tolerance of human gastric carcinoma cell line BGC-823 induced by cisplatin[J];Journal of Dalian Medical University;2005-01
2 HE Jian-ming, LIANG Hou-jie, BIAN Zhi-heng, HU Shao-yi (Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Mechanisms of cell-cell adhesion dependent multicellular drug resistance of pulmonary adenocarcinoma cell line A549[J];Acta Academiae Medicinae Militaris Tertiae;2004-24
3 LIU Da Qing 1, SITU Zhen Qiang 1, ZHOU Shu Xia 1, CAO Yun Xin 3, XU Yan Ming 2, YU Cui Juan 2, WANG Cheng Ji 2, YANG An Gang 2 1Department of Oral and Maxillofacial Surgery, Stomatological College, 2Department of Biochemistry and Molecular Biology, 3Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi’an 710033, China;Apoptosis of mucoepidermoid carcinoma cells induced by recombinant human tumor necrosis factor-α[J];Journal of The Fourth Military Medical University;2004-01
4 MA Qiang,ZHANG Fang-Xin,KANG Sheng-Chao,CHEN Jia-YuDepartment of Gastroenterology,Lanzhou General Hospital,Lanzhou Military Area Command,Lanzhou 730050,China;Changes of hTERT mRNA during the process of multidrug resistance by adriamycin in human colon carcinoma LoVo cells[J];Journal of the Fourth Military Medical University;2006-15
5 GAO Hong-quan,LIV Yue-guang,WANG Yu.Mudanjiang Medical College,Mudanjiang 157011 China;Oxymatrine induces apoptosis in ovarian cancer cell line HO8910[J];Anatomy Research;2007-03
6 LOU Jiang-yan, PENG Zhi-lan, ZHENG Ying, HE Bin. Department of Obsterics and Gynecology,West China Second Hospital, Sichuan University, Chengdu 610041, China;Improve the Chemosensitivity of Resistant Ovarian Cancer Cell by Small RNA Interference[J];Journal of Sichuan University(Medical Science Edition);2008-03
7 LIU Rui-bao, XU Ke, LI Feng, et al. Department of Radiology, Third Hospital, Harbin Medical University, Harbin 150040, China;Establishment of the MDR model in rabbit liver with VX2[J];Journal of Interventional Radiology;2004-04
8 Li yan, Wang Chunmei, Huang Xiaofeng,et al.Department of oral Biology, Stomatological Collegy, Fourth Military Medical University1,Xi'an 710032,China;Characterzation of mitochondria in multidrug resistant cell clone MEC-1/5-FU of human salivary gland mucoepidermoid carcinoma[J];Journal of Clinical Stomatology;2003-04
9 Wang Ying Mudanjiang Medical College 157011;STUDIES OF GFW ON INDUCING APOPTOSIS IN OVARIAN CANCER CELL LINES HO8910[J];Journal of Mudanjiang Medical College;2003-06
10 Wangying et al. Mudanjiang Medical College 157011;OXYMATRINE INDUCES APOPTOSIS AND EFFECT OF CELL CYCLE IN OVARIAN CANCER CELL LINES HO8910[J];Journal of Mudanjiang Medical College;2004-01
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 ZHANG Hong Xin 1,WANG Zhi Min 1,GUO Wei Ping 1,WANG Yi Qing 1,LIU Yan 2,LI Wen Xian 1,NI Dai Hui 1,GUAN Yan 1,GAO Wei 3 1Department of Interventional Radiology, 2Department of Ophthalmology, 3Department of Gastroenterology,Tan;Establishment of human multidrug-resistant hepatocellular carcinoma cell line(7721/Adm)[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-04
2 Li Jiaping 1,Bian Duhong 2.1.Department of Obstetrics and Gynecology,Chengdu Third People's Hospital,Chengdu 610031;2.Department of Obstetrics and Gynecology,First Clinical Hospital of Chongqing Medical University;Dervation and biological characterization of multidrug resistant human ovarian carcinoma cell line.[J];Current Advances In Obstetrics and Gynecology;2001-01
3 Huang Wei; Li Li; Tang Dongping; (Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021.);ESTABLISHING OF A NEW CISPLATIN-RESISTANT CELL LINE AND STUDING OF ITS MECHANISM CHARACTERIZATION OF DRUG-RESISTANCE[J];CHINA ONCOLOGY;1998-03
4 Zhou Youzhen , Chen Huizhen ,Yang Qinyi , et al (Second Affiliated Hospital ,Hubei Med- ical University ,Wuhan , 430071);Establishment of human ovarian cancer cisplatin- resistant cell line COC1 /DDP and its mechanism of resistance[J];National Medical Journal of China;1996-09
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